ISO 12966-2:2017 PDF

Scope: This document specifies methods of preparing the methyl esters of fatty acids. It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements, four methylation methods are specified, namely: a) a “rapid” transmethylation procedure under alkaline conditions; b) a “general” transmethylation/methylation procedure under sequential alkaline and acid conditions; c) a BF3 transmethylation procedure; d) an alternative procedure using acid-catalysed transmethylation of glycerides. Methyl esters so produced are used in various analytical procedures requiring such derivatives, e.g. gas-liquid chromatography (GLC), thin-layer chromatography (TLC) and infrared spectrometry (IR). Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.

ISO 12966-2:2017 Referenced Document

ISO 1042:1998 Laboratory glassware - One-mark volumetric flasks
ISO 3696:1987 Water for analytical laboratory use; Specification and test methods
ISO 4799:1978 Laboratory glassware; Condensers
ISO 4800:1998 Laboratory glassware - Separating funnels and dropping funnels
ISO 661:2003 Animal and vegetable fats and oils - Preparation of test sample
ISO 78-2:1999 Chemistry - Layouts for standards - Part 2: Methods of chemical analysis
ISO 835:2007 Laboratory glassware - Graduated pipettes
ISO 8655-2:2002 Piston-operated volumetric apparatus - Part 2: Piston pipettes

2017 ISO 12966-2:2017 Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 2: Preparation of methyl esters of fatty acids
2011 ISO 12966-2:2011 Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 2: Preparation of methyl esters of fatty acids