ISO 12966-2:2011 Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 2: Preparation of methyl esters of fatty acids
This part of ISO 12966 specifies methods of preparing the methyl esters of fatty acids.
It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids
and soaps. To cover different requirements four methylation methods are specified, namely:
a) a “rapid” transmethylation procedure under alkaline conditions;
b) a “general” transmethylation/methylation procedure under sequential alkaline and acid conditions;
c) a BF transmethylation procedure;
d) an alternative procedure using acid-catalysed transmethylation of glycerides.
Methyl esters so produced are used in various analytical procedures requiring such derivatives, e.g. gas-liquid
chromatography (GLC), thin-layer chromatography (TLC), and infrared spectrometry (IR).
ISO 12966-2:2011 Referenced Document
ISO 661 Animal and vegetable fats and oils - Preparation of test sample
ISO 12966-2:2011 history
2017ISO 12966-2:2017 Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 2: Preparation of methyl esters of fatty acids
2011ISO 12966-2:2011 Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 2: Preparation of methyl esters of fatty acids