ASTM D6469-17
Standard Guide for Microbial Contamination in Fuels and Fuel Systems

Standard No.

ASTM D6469-17

Release Date

2017

Published By

American Society for Testing and Materials (ASTM)

Status

Be replaced

Replace By

ASTM D6469-20

Latest

ASTM D6469-20

Scope

5.1 This guide provides information addressing the conditions that lead to fuel microbial contamination and biodegradation and the general characteristics of and strategies for controlling microbial contamination. It compliments and amplifies information provided in Practice D4418 on handling gas-turbine fuels. More detailed information may be found in the IP Guidelines and in ASTM Manual 47.

5.2 This guide focuses on microbial contamination in refined petroleum products and product handling systems. Uncontrolled microbial contamination in fuels and fuel systems remains a largely unrecognized but costly problem at all stages of the petroleum industry from crude oil production through fleet operations and consumer use. This guide introduces the fundamental concepts of fuel microbiology and biodeterioration control.

5.3 This guide provides personnel who are responsible for fuel and fuel system stewardship with the background necessary to make informed decisions regarding the possible economic or safety, or both, impact of microbial contamination in their products or systems.

1.1 This guide provides personnel who have a limited microbiological background with an understanding of the symptoms, occurrence, and consequences of chronic microbial contamination. The guide also suggests means for detection and control of microbial contamination in fuels and fuel systems. This guide applies primarily to gasoline, aviation, boiler, industrial gas turbine, diesel, marine, furnace fuels and blend stocks (see Specifications D396, D910, D975, D1655, D2069, D2880, D3699, D4814, D6227, and D6751), and fuel systems. However, the principles discussed herein also apply generally to crude oil and all liquid petroleum fuels. ASTM Manual 472 provides a more detailed treatment of the concepts introduced in this guide; it also provides a compilation of all of the standards referenced herein that are not found in the Annual Book of ASTM Standards, Section Five on Petroleum Products and Lubricants.

1.2 This guide is not a compilation of all of the concepts and terminology used by microbiologists, but it does provide a general understanding of microbial fuel contamination.

1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

1.5x00a......

ASTM D6469-17 Referenced Document

• ASTM D1067 Standard Test Methods for Acidity or Alkalinity of Water
• ASTM D1126 Standard Test Method for Hardness in Water
• ASTM D1293 Standard Test Methods for pH of Water
• ASTM D1298 Standard Test Method for Density, Relative Density (Specific Gravity), or API Gravity of Crude Petroleum and Liquid Petroleum Products by Hydrometer Method
• ASTM D130 Standard Test Method for Detection of Copper Corrosion from Petroleum Products by the Copper Strip Tarnish Test
• ASTM D1331 Standard Test Methods for Surface and Interfacial Tension of Solutions of Surface-Active Agents
• ASTM D1426 Standard Test Methods for Ammonia Nitrogen In Water
• ASTM D1655 Standard Specification for Aviation Turbine Fuels
• ASTM D1744 Standard Test Method for Determination of Water in Liquid Petroleum Products by Karl Fischer Reagent
• ASTM D1976 Standard Test Method for Elements in Water by Inductively-Coupled Argon Plasma Atomic Emission Spectroscopy
• ASTM D2068 Standard Test Method for Filter Blocking Tendency of Distillate Fuel Oils
• ASTM D2069 
• ASTM D2274 Standard Test Method for Oxidation Stability of Distillate Fuel Oil (Accelerated Method)
• ASTM D2276 Standard Test Method for Particulate Contaminant in Aviation Fuel by Line Sampling
• ASTM D2880 Standard Specification for Gas Turbine Fuel Oils
• ASTM D3240 Standard Test Method for Undissolved Water In Aviation Turbine Fuels
• ASTM D3241 Standard Test Method for Thermal Oxidation Stability of Aviation Turbine Fuels (JFTOT Procedure)
• ASTM D3242 Standard Test Method for Acidity in Aviation Turbine Fuel
• ASTM D3325 Standard Practice for Preservation of Waterborne Oil Samples
• ASTM D3326 Standard Practice for Preparation of Samples for Identification of Waterborne Oils
• ASTM D3328 Standard Test Methods for Comparison of Waterborne Petroleum Oils by Gas Chromatography
• ASTM D3414 Standard Test Method for Comparison of Waterborne Petroleum Oils by Infrared Spectroscopy
• ASTM D3699 Standard Specification for Kerosine
• ASTM D3867 Standard Test Methods for Nitrite-Nitrate in Water
• ASTM D3870 Standard Practice for Establishing Performance Characteristics for Colony Counting Methods in Microbiology
• ASTM D396 Standard Specification for Fuel Oils
• ASTM D4012 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
• ASTM D4057 Standard Practice for Manual Sampling of Petroleum and Petroleum Products
• ASTM D4176 Standard Test Method for Free Water and Particulate Contamination in Distillate Fuels (Visual Inspection Procedures)
• ASTM D4412 Standard Test Methods for Sulfate-Reducing Bacteria in Water and Water-Formed Deposits
• ASTM D4418 Standard Practice for Receipt, Storage, and Handling of Fuels for Gas Turbines
• ASTM D445 Standard Test Method for Kinematic Viscosity of Transparent and Opaque Liquids (the Calculation of Dynamic Viscosity)
• ASTM D4454 Standard Test Method for Simultaneous Enumeration of Total and Respiring Bacteria in Aquatic Systems by Microscopy
• ASTM D4814 Standard Specification for Automotive Spark-Ignition Engine Fuel
• ASTM D4840 Standard Guide for Sample Chain-of-Custody Procedures
• ASTM D4860 Standard Test Method for Free Water and Particulate Contamination in Mid-Distillate Fuels (Clear and Bright Numerical Rating)
• ASTM D4870 Standard Test Method for Determination of Total Sediment in Residual Fuels
• ASTM D4952 Standard Test Method for Qualitative Analysis for Active Sulfur Species in Fuels and Solvents (Doctor Test)
• ASTM D515 Standard test method for dust abrasion of granular activated carbon
• ASTM D5304 Standard Test Method for Assessing Middle Distillate Fuel Storage Stability by Oxygen Overpressure
• ASTM D5452 Standard Test Method for Particulate Contamination in Aviation Fuels by Laboratory Filtration
• ASTM D6217 Standard Test Method for Particulate Contamination in Middle Distillate Fuels by Laboratory Filtration
• ASTM D6227 Standard Specification for Grade 82 Unleaded Aviation Gasoline
• ASTM D6426 Standard Test Method for Determining Filterability of Distillate Fuel Oils
• ASTM D664 Standard Test Method for Acid Number of Petroleum Products by Potentiometric Titration
• ASTM D6751 Standard Specification for Biodiesel Fuel Blendstock (B100) for Middle Distillate Fuels
• ASTM D6974 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
• ASTM D7463 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water
• ASTM D7464 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
• ASTM D7687 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration (Standard + Redline PDF Bundle)
• ASTM D7978 Standard Test Method for Determination of the Viable Aerobic Microbial Content of Fuels and Associated Water—Thixotropic Gel Culture Method
• ASTM D8070 Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay
• ASTM D888 Standard Test Methods for Dissolved Oxygen in Water
• ASTM D910 Standard Specification for Aviation Gasolines
• ASTM D974 Standard Test Method for Acid and Base Number by Color-Indicator Titration
• ASTM D975 Standard Specification for Diesel Fuel Oils
• ASTM E1259 Standard Test Method for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390176;C
• ASTM E1326 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
• ASTM E177 Standard Practice for Use of the Terms Precision and Bias in ASTM Test Methods

ASTM D6469-17 history

• 2020 ASTM D6469-20 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
• 2017 ASTM D6469-17 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
• 2014 ASTM D6469-14 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
• 2012 ASTM D6469-12 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
• 2011 ASTM D6469-11 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
• 2008 ASTM D6469-08e1 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
• 2008 ASTM D6469-08 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
• 2004 ASTM D6469-04 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
• 1999 ASTM D6469-99 Standard Guide for Microbial Contamination in Fuels and Fuel Systems