ASTM D6974-20
Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures

Standard No.

ASTM D6974-20

Release Date

2020

Published By

American Society for Testing and Materials (ASTM)

Latest

ASTM D6974-20

Scope

1.1 This practice covers a membrane filter (MF) procedure for the detection and enumeration of Heterotrophic bacteria (HPC) and fungi in liquid fuels with kinematic viscosities ≤24 mm2 · s-1 at ambient temperature. 1.2 This quantitative practice is drawn largely from IP Method 385 and Test Method D5259. 1.3 This test may be performed either in the field or in the laboratory. 1.4 The ability of individual microbes to form colonies on specific growth media depends on the taxonomy and physiological state of the microbes to be enumerated, the chemistry of the growth medium, and incubation conditions. Consequently, test results should not be interpreted as absolute values. Rather they should be used as part of a diagnostic or condition monitoring effort that includes other test parameters, in accordance with Guide D6469. 1.5 This practice offers alternative options for delivering fuel sample microbes to the filter membrane, volumes or dilutions filtered, growth media used to cultivate fuel-borne microbes, and incubation temperatures. This flexibility is offered to facilitate diagnostic efforts. When this practice is used as part of a condition monitoring program, a single procedure should be used consistently. 1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ASTM D6974-20 Referenced Document

• ASTM D1129 Standard Terminology Relating to Water
• ASTM D1193 Standard Specification for Reagent Water
• ASTM D4175 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants*， 2023-07-01 Update
• ASTM D5259 Standard Test Method for Isolation and Enumeration of Enterococci from Water by the Membrane Filter Procedure
• ASTM D6426 Standard Test Method for Determining Filterability of Distillate Fuel Oils
• ASTM D6469 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
• ASTM D7463 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water*， 2021-04-01 Update
• ASTM D7464 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
• ASTM E1326 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
• ASTM F1094 Standard Test Methods for Microbiological Monitoring of Water Used for Processing Electron and Microelectronic Devices by Direct Pressure Tap Sampling Valve and by the Presterilized Plastic Bag Method

ASTM D6974-20 history

• 2020 ASTM D6974-20 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
• 2016 ASTM D6974-16 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels&x2014;Filtration and Culture Procedures
• 2009 ASTM D6974-09(2013)e2 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
• 2009 ASTM D6974-09(2013)e1 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuelsmdash;Filtration and Culture Procedures
• 2009 ASTM D6974-09(2013) Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuelsmdash;Filtration and Culture Procedures
• 2009 ASTM D6974-09 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels8212;Filtration and Culture Procedures
• 2004 ASTM D6974-04 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels8212;Filtration and Culture Procedures
• 2003 ASTM D6974-03 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels-Filtration and Culture Procedures