ASTM D8070-23
Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay

Standard No.

ASTM D8070-23

Release Date

2023

Published By

American Society for Testing and Materials (ASTM)

Latest

ASTM D8070-23

Scope

1.1 This test method describes a procedure that can be used in the field or in a laboratory to detect antigens indicative of microbial contamination in liquid fuels, including those blended with synthesized hydrocarbons or biofuels, with kinematic viscosities (at 40 °C) of ≤24 mm2 s–1 (for example, Specifications D396, D975, and D1655) and in fuel-associated water. 1.1.1 This test method has been validated by an ILS for a range of middle distillate fuels meeting Specification D1655, EN590, Specification D975, and ISO 8217:2012. 1.2 This test method semi-quantitatively assesses the concentration of specific antigens generated by commonly recovered, fuel-associated, aerobic microorganisms during active growth in fuel systems. 1.2.1 A proprietary formulation of antibodies and antibody mixtures is used to detect three types of microbial antigen contamination: antigens generally found in aerobic bacteria, antigens generally present in common fungi (yeast and molds), and an antigen that is characteristic of Hormoconis resinae (the fungus most commonly associated with fuel biodeterioration). 1.2.2 Although the antibodies and antibody mixtures are characteristic of diverse types of bacteria and fungi, it is unlikely that they are universal. Recognizing that for every microbe that has been isolated and characterized, it is likely that there are a billion that have not. Consequently, as is the case with all microbiological test methods, this test method does not purport to detect 100 % of the microbes present in a fuel or fuel-associated water sample. 1.3 For each of the three sets of antigen detected (H. resinae, common fungi, and aerobic bacteria), the test detects whether the antigen concentration present is within set ranges representing negligible, moderate, or heavy microbial contamination. 1.3.1 For fuel specimens, the antigen concentration ranges detected are <150 µg ⁄L (negligible), 150 µg ⁄L to 750 µg ⁄L (moderate), and >750 µg ⁄L (heavy). 1.3.2 For specimens of water associated with fuel, the antigen concentration ranges detected are <33 µg ⁄mL (negligible), 33 µg ⁄mL to 166 µg ⁄mL (moderate), and >166 µg ⁄mL (heavy). 1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. For a specific hazard statement, see Section 8. 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ASTM D8070-23 Referenced Document

• ASTM D1129 Standard Terminology Relating to Water
• ASTM D1655 Standard Specification for Aviation Turbine Fuels
• ASTM D396 Standard Specification for Fuel Oils
• ASTM D4175 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
• ASTM D4176 Standard Test Method for Free Water and Particulate Contamination in Distillate Fuels (Visual Inspection Procedures)
• ASTM D6469 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
• ASTM D7464 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
• ASTM D7687 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration (Standard + Redline PDF Bundle)
• ASTM D7847 Standard Guide for Interlaboratory Studies for Microbiological Test Methods
• ASTM D7978 Standard Test Method for Determination of the Viable Aerobic Microbial Content of Fuels and Associated Water—Thixotropic Gel Culture Method
• ASTM D975 Standard Specification for Diesel Fuel Oils
• ASTM E1326 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
• ASTM E2756 Standard Terminology Relating to Antimicrobial and Antiviral Agents

ASTM D8070-23 history

• 2023 ASTM D8070-23 Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay
• 2021 ASTM D8070-21 Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay
• 2020 ASTM D8070-20 Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay
• 2019 ASTM D8070-19a Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay
• 2019 ASTM D8070-19 Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay
• 2016 ASTM D8070-16 Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay