EN ISO 12966-3:2016 Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 3: Preparation of methyl esters using trimethylsulfonium hydroxide (TMSH)
This part of the International Standard ISO 12966 specifies a rapid process for the base-catalyzed transesterification of fats and oils with trimethylsulfonium hydroxide (TMSH) for the production of fatty acid methyl esters. The method is applicable exclusively to the production of methyl esters of fats or oils for gas-liquid chromatography (GLC) analysis. It can be used with all fats and oils, except for fats from milk and milk products. Isomerization of unsaturated fatty acids only occurs to a small extent and the amount of isomerized fatty acids present is at the limit of quantification. Because isomerization occurs, the process is not recommended for conjugated linoleic acid (CLA). Free fatty acids are only esterified to around 70% to 80%. Side reactions may occur with conjugated cyclopropyl and cyclopropenyl fatty acids, but these do not interfere with the determination of the fatty acids. NOTE This part of ISO 12966 is based on the German Standard Method C-VI 11e (98).
EN ISO 12966-3:2016 Referenced Document
ISO 1042:1998 Laboratory glassware - One-mark volumetric flasks
ISO 5555:2001 Animal and vegetable fats and oils - Sampling
ISO 661:2003 Animal and vegetable fats and oils - Preparation of test sample
ISO 835:2007 Laboratory glassware - Graduated pipettes
EN ISO 12966-3:2016 history
2016EN ISO 12966-3:2016 Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 3: Preparation of methyl esters using trimethylsulfonium hydroxide (TMSH)
2009EN ISO 12966-3:2009 Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 3: Preparation of methyl esters using trimethylsulfonium hydroxide (TMSH)