ASTM D5465-16
Standard Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods

Standard No.

ASTM D5465-16

Release Date

2016

Published By

American Society for Testing and Materials (ASTM)

Status

Be replaced

Replace By

ASTM D5465-16(2020)

Latest

ASTM D5465-16(2020)

Scope

4.1 Numerous ASTM test methods and practices (for example: Test Methods D5259 and D5392, and Practices D6974 and E2563) report colony counts as their measured parameter.

4.2 These practices provide a uniform set of counting, calculating, and reporting procedures for ASTM test methods in microbiology.

	Section
A—Counting Colonies on Membrane Filters	6
B—Counting Colonies on Pour Plates	7
C—Counting Colonies on Spread Plates	8

4.3 The counting rules provide a best attainable estimate of microorganisms in the sample, since the samples cannot be held and reanalyzed at a later date.

1.1 These practices cover recommended procedures for counting colonies and reporting colony-forming units (CFU) on membrane filters (MF) and standard pour and spread plates.

1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

ASTM D5465-16 Referenced Document

• ASTM D1129 Standard Terminology Relating to Water
• ASTM D5259 Standard Test Method for Isolation and Enumeration of Enterococci from Water by the Membrane Filter Procedure
• ASTM D5392 Standard Test Method for Isolation and Enumeration ofEscherichia Coli in Water by the Two-Step Membrane Filter Procedure
• ASTM D6161 Standard Terminology Used for Crossflow Microfiltration, Ultrafiltration, Nanofiltration, and Reverse Osmosis Membrane Processes
• ASTM D6974 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
• ASTM E2563 Standard Practice for Enumeration of Non-Tuberculosis Mycobacteria in Aqueous Metalworking Fluids by Plate Count Method

ASTM D5465-16 history

• 2020 ASTM D5465-16(2020) Standard Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
• 2016 ASTM D5465-16 Standard Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
• 1993 ASTM D5465-93(2012) Standard Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
• 1993 ASTM D5465-93(2004) Standard Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
• 1993 ASTM D5465-93(1998) Standard Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods