ISO 12966-1:2014
Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 1: Guidelines on modern gas chromatography of fatty acid methyl esters

Standard No.

ISO 12966-1:2014

Release Date

2014

Published By

International Organization for Standardization (ISO)

Latest

ISO 12966-1:2014

Scope

This part ofISO 12966 gives an overview ofthe gas chromatographic determination of fatty acids, free and bound, in animal and vegetable fats and oils following their conversion to fatty acid methyl esters (FAMEsS). The qualitative and duantitative determination of the_ composition of fatty acids by gas lidquid chromatography (GLCJ] is a widely used application in lipid analysis. It is used for the characterization of fats and oils, or fatty foodstuffs after the extraction ofthe oil from the matrix. The bound fatty acids of the triacylglycerols (TAGS) and, depending on the esterification method, the free fatty acids (FFA) and other lipids, are converted to fatty acid methyl esters (FAMESsj, which are determined by capillary gas chromatography. Depending on the number of different fatty acids (theoretically more than 50 different fatty acids can be present)] capillary columns with a length of 10 m to 100 m are used for a separation. The GLC of FAMEs is applicable to all natural and synthetic mixtures of tri-, di- and monoacylglycerols， to fatty acid esters, free fatty acids, soaps and other fatty compounds. With this Suite of standards， FAMEs from C4to C26 can be determined, including saturated fatty acid methyl esters, cis- and trans- monounsaturated fatty acid methyl esters, and cis- and trans-polyunsaturated fatty acid methyl esters. For the determination of short chain fatty acids, isopropyl and butyl esters are often used so as to avoid interferences with the Solvent peak and in order to reduce differences in detector responses.

ISO 12966-1:2014 Referenced Document

• ISO 12966-2:2011 Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 2: Preparation of methyl esters of fatty acids
• ISO 12966-3:2009 Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 3: Preparation of methyl esters using trimethylsulfonium hydroxide (TMSH)
• ISO 12966-4 Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 4: Determination by capillary gas chromatography*， 2015-06-01 Update

ISO 12966-1:2014 history

• 2014 ISO 12966-1:2014 Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 1: Guidelines on modern gas chromatography of fatty acid methyl esters