5.1 An important goal of aquatic toxicology is to determine the effects of toxic compounds on species that play a central role in aquatic communities. Rotifers have a major impact on several important ecological processes in freshwater and coastal marine environments. As filter-feeders on phytoplankton and bacteria, rotifers exert substantial grazing pressure that at times exceeds that of the larger crustacean zooplankton (1, 2).3 Rotifer grazing on phytoplankton is highly selective (2-4) and can influence phytoplankton composition, the coexistence of competitors, and overall water quality (5). The contribution of rotifers to the secondary production of many aquatic communities is substantial (6-9). In fresh water, rotifers often account for the major fraction of zooplankton biomass at certain times of the year (10, 11) . Rotifers and other zooplankton are a significant food source for many larval fish, planktivorous adult fish (12, 13), and several invertebrate predators (14-16) . The high metabolic rates of rotifers contribute to their role in nutrient cycling, which might make rotifers more important than crustaceans in certain communities (17, 18) .
5.2 In addition to their important ecological role in aquatic communities, rotifers are attractive organisms for toxicological studies because an extensive database exists on the basic biology of this group. Techniques have been published for the culture of many rotifer species (3, 19). The rotifer life cycle is well defined (20,
ASTM E1440-91(2012) Referenced Document
ASTM E1440-91(2012) history
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