ASTM E729-96(2014)
Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians

Standard No.

ASTM E729-96(2014)

Release Date

1996

Published By

American Society for Testing and Materials (ASTM)

Status

Be replaced

Replace By

ASTM E729-23

Latest

ASTM E729-23e1

Scope

5.1 An acute toxicity test is conducted to obtain information concerning the immediate effects on test organisms of a short-term exposure to a test material under specific experimental conditions. An acute toxicity test does not provide information about whether delayed effects will occur, although a post-exposure observation period, with appropriate feeding, if necessary, might provide such information.

5.2 Results of acute toxicity tests might be used to predict acute effects likely to occur on aquatic organisms in field situations as a result of exposure under comparable conditions, except that (1) motile organisms might avoid exposure when possible, and (2) toxicity to benthic organisms might be dependent on sorption or settling of the test material onto the substrate.

5.3 Results of acute tests might be used to compare the acute sensitivities of different species and the acute toxicities of different test materials, and to study the effects of various environmental factors on results of such tests.

5.4 Results of acute toxicity tests might be an important consideration when assessing the hazards of materials to aquatic organisms (see Guide E1023) or when deriving water quality criteria for aquatic organisms (2).

5.5 Results of acute toxicity tests might be useful for studying the biological availability of, and structure-activity relationships between, test materials.

5.6 Results of acute toxicity tests will depend on the temperature, composition of the dilution water, condition of the test organisms, exposure technique, and other factors.

1.1 This guide (1)2 describes procedures for obtaining laboratory data concerning the adverse effects (for example, lethality and immobility) of a test material added to dilution water, but not to food, on certain species of freshwater and saltwater fishes, macroinvertebrates, and amphibians during 2 to 8-day exposures, depending on the species. These procedures will probably be useful for conducting acute toxicity tests with many other aquatic species, although modifications might be necessary.

1.2 Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting acute tests.

1.3 This guide describes tes......

ASTM E729-96(2014) Referenced Document

• ASTM E1023 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
• ASTM E1191 Standard Guide for Conducting Life-Cycle Toxicity Tests with Saltwater Mysids*， 1997-02-10 Update
• ASTM E1192 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians*， 1997-04-20 Update
• ASTM E1203 Standard Practice for Using Brine Shrimp Nauplii as Food for Test Animals in Aquatic Toxicology *， 1998-04-20 Update
• ASTM E1563 Standard Guide for Conducting Static Acute Toxicity Tests with Echinoid Embryos*， 1998-04-20 Update
• ASTM E1604 Standard Guide for Behavioral Testing in Aquatic Toxicology
• ASTM E724 Standard Guide for Conducting Static Acute Toxicity Tests Starting with Embryos of Four Species of Saltwater Bivalve Molluscs*， 1998-04-20 Update
• ASTM E943 Standard Terminology Relating to Biological Effects and Environmental Fate*， 2023-06-01 Update
• IEEE/ASTM SI 10 American National Standard for Metric Practice

ASTM E729-96(2014) history

• 2023 ASTM E729-23e1 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
• 2023 ASTM E729-23 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
• 1996 ASTM E729-96(2014) Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
• 1996 ASTM E729-96(2007) Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
• 1996 ASTM E729-96(2002) Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
• 1996 ASTM E729-96 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians