A microcosm test is conducted to obtain information concerning toxicity or other effects of a test material on the interactions among three trophic levels (primary, secondary, and detrital) and the competitive interactions within each trophic level. As with most natural aquatic ecosystems, the microcosms depend upon algal production (primary production) to support the grazer trophic level (secondary production), which along with the microbial community are primarily responsible for the nutrient recycling necessary to sustain primary production. Microcosm initial condition includes some detritus (chitin and cellulose) and additional detritus is produced by the system. The microcosms include ecologically important processes and organisms representative of ponds and lakes, but are non-site specific.
The species used are easy to culture in the laboratory and some are routinely used for single species toxicity tests (Guide E729; Practice D3978, Guides E1192 and E1193). Presumably acute toxicity test results with some of these species would be available prior to the decision to undertake the microcosm test. If available, single species toxicity results would aid in distinguishing between indirect and direct effects.
These procedures are based mostly on previously published methods (4-6), interlaboratory testing (7-10), intermediate studies (11-22), statistical studies (23-25) and mathematical simulation results (26). Newer studies on jet fuels have been reported (27)(See 15.1 for multivariate statistical analyses) and on the implications of multispecies testing for pesticide registration (28). Environmental Protection Agency, (EPA) and Food and Drug Administration, (FDA) published similar microcosm tests (29). The methods described here were used to determine the criteria for Acceptable Tests (Section 16).
Concurrent to measuring the ecological effects, it is advisable to measure the concentration of the parent test chemical, and if possible, the transformation products ((30) see Section 12). The concentrations can be measured on either the same microcosms or on concurrent replicates. Information on the chemical concentrations of parent material and transformation products would aid in the assessment of chemical persistence, exposure, accumulation, and in interpreting, if recovery is associated with chemical degradation or biological adaptation. This protocol deals only with ecological effects, because the techniques for fate studies are in general usage.
In the microcosm, as in natural ecosystems, a population must be able to obtain its requirements from the products of other trophic levels, to maintain a birth rate equal to or greater than its death rate, and to support populations of organisms that will remove its waste products. As in natural ecosystems, several organisms might be capable of fulfilling the same function, and shifts in species dominance can occur without disruption of an ecological process. However, species that are “ecological equivalents” in one function might not be “equivalent” in other functions; for example, a filamentous alga and a single cell alga might equally produce O2, remove NO3, NH3, and ..........
Copyright ©2024 All Rights Reserved