This International Standard specifies a method for the quantitative determination of the total sterols content by means of an enzymatic staining test. The method is applicable to free and esterified sterols in animal and vegetable fats and oils, fatty foods and related products. The determination is applicable to sample quantities of 1 g to 2 g of fat.
The method is not applicable to dark coloured fats and oils, e.g. crude palm oil. The enzyme is not specific for cholesterol, but also oxidizes other 3-hydroxysterols. The method has not been tested for products fortified with sterols at higher levels.
NOTE The method is technically equivalent to IUPAC method 2.404 and DGF standard method F-III 2 (91).
BS EN ISO 11702:2009 Referenced Document
ISO 3696 Water for analytical laboratory use; Specification and test methods
ISO 661 Animal and vegetable fats and oils - Preparation of test sample
BS EN ISO 11702:2009 history
2016BS EN ISO 11702:2016 Animal and vegetable fats and oils. Enzymatic determination of total sterols content
2010BS EN ISO 11702:2009 Animal and vegetable fats and oils - Enzymatic determination of total sterols content