1.1 This test method covers procedures for testing freshwater organisms in the laboratory to evaluate the toxicity of contaminants associated with whole sediments. Sediments may be collected from the field or spiked with compounds in the laboratory.
1.1.1 Test methods are described for two toxicity test organisms, the amphipod Hyalella azteca ( H. azteca) (see ) and the midge Chironomus dilutus (formerly known as C. tentans; Shobanov et al. 1999.
Morphological comparison of populations of Chironomus (Camptochironomus) tentans(Fabricius) from Europe, Asia, and North America have confirmed cytogenetic evidence that two distinct species inhabit the Palearctic and Nearctic under this name. The Palearctic species is the true C. tentans and the Nearctic populations constitute a new species described under the name Chironomus (Camptochironomus) dilutus (Shobanov et al. 1999
1.1.2 Guidance for conducting sediment toxicity tests is outlined in for Chironomus riparius, in for Daphnia magna and Ceriodaphnia dubia, in for Hexagenia spp., in for Tubifex tubifex, and in for the Diporeia spp. Guidance is also provided in for conducting long-term sediment toxicity tests with H. azteca by measuring effects on survival, growth, and reproduction. Guidance is also provided in for conducting long-term sediment toxicity tests with C. dilutus by measuring effects on survival, growth, emergence, and reproduction. outlines the data that will be needed before test methods are developed from the guidance outlined in to for these test organisms. General procedures described in Sections for sediment testing with H. azteca and C. dilutus are also applicable for sediment testing with the test organisms described in to .
1.2 Procedures outlined in this test method are based primarily on procedures described in the United States Environmental Protection Agency (USEPA)
1.3 Additional research and methods development are now in progress to: (1) evaluate additional test organisms, (2) further evaluate the use of formulated sediment, (3) refine sediment dilution procedures, (4) refine sediment toxicity identification evaluation (TIE) procedures
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