International Organization for Standardization (ISO)
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ISO 13884:2003
Scope
This International Standard specifies a method for the accurate determination of isolated trans bonds in natural or processed long-chain fatty acids, fatty acid esters and triglycerides with trans isomer levels > 5 %.
The method is not applicable, or is applicable only with specific precautions, to
— fats and oils containing high levels (over 5 %) of conjugated unsaturation (e.g. tung oil),
— materials containing functional groups which modify the intensity of the C-H deformation about the trans double bond [e.g. castor oil containing ricinoleic acid or its geometrical isomer, ricinelaidic acid (12-hydroxy-9-octadecenoic acid)],
— mixed triglycerides having long- and short-chain moieties (such as diacetostearin), or in general,
— any materials containing constituents which have functional groups which give rise to specific absorption bands at or sufficiently close to interfere with the 966 cm band of the C-H deformation of the isolated trans double bond.
NOTE Dienes, such as cis-trans and trans-trans dienes, can affect the calibration.
For accurate determinations on materials with trans levels below 5 %, a standardized capillary GLC method is recommended (for example, see ISO 15304).
ISO 13884:2003 Referenced Document
ISO 5509 Animal and vegetable fats and oils - Preparation of methyl esters of fatty acids
ISO 661 Animal and vegetable fats and oils - Preparation of test sample
ISO 13884:2003 history
2003ISO 13884:2003 Animal and vegetable fats and oils - Determination of isolated trans isomers by infrared spectrometry