ASTM E1415-22
Standard Guide for Conducting Static Toxicity Tests With Lemna gibba G3

Standard No.

ASTM E1415-22

Release Date

2022

Published By

American Society for Testing and Materials (ASTM)

Latest

ASTM E1415-22

Scope

1.1 This guide describes procedures for obtaining laboratory data concerning the adverse effects of a text material added to growth medium on a certain species of duckweed (Lemna gibba G3) during a 7-day exposure using the static technique. These procedures will probably be useful for conducting toxicity tests with other species of duckweed and other floating vascular plants, although modifications might be necessary. 1.2 Special needs or circumstances might also justify modification of this standard. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting tests with duckweed. 1.3 The procedures in this guide are applicable to most chemicals, either individually or in formulations, commercial products, or known mixtures. With appropriate modifications these procedures can be used to conduct tests on temperature and pH and on such other materials as aqueous effluents (see also Guide E1192), leachates, oils, particulate matter, sediments and surface waters. These procedures do not specifically address effluents because to date there is little experience using duckweeds in effluent testing and such tests may pose problems with acclimation of the test organisms to the receiving water. Static tests might not be applicable to materials that have a high oxygen demand, are highly volatile, are rapidly biologically or chemically transformed in aqueous solution, or are removed from test solutions in substantial quantities by the test chambers or organisms during the test. 1.4 Results of toxicity tests performed using the procedures in this guide should usually be reported in terms of the 7-day IC50 based on inhibition of growth. In some situations it might only be necessary to determine whether a specific concentration unacceptably affects the growth of the test species or whether the IC50 is above or below a specific concentration. Another end point that may be calculated is the no observed effect concentration (NOEC). 1.5 The sections of this guide appear as follows: Title Section Referenced Documents 2 Terminology 3 Summary of Guide 4 Significance and Use 5 Hazards 6 Apparatus 7 Facilities 7.1 Test Chambers 7.2 Cleaning 7.3 Acceptability 7.4 Growth Medium 8 Test Material 9 General 9.1 Stock Solution 9.2 Test Concentration(s) 9.3 Test Organisms 10 Species 10.1 Source 10.2 Stock Culture 10.3 Procedure 11 Experimental Design 11.1 Temperature 11.2 Illumination 11.3 Beginning the Test 11.4 Duration of Test 11.5 Biological Data 11.6 Other Measurements 11.7 Analytical Methodology 12 Acceptability of Test 13 Calculation of Results 14 Report 15 1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 6. 1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. 1 This guide is under the jurisdiction of ASTM Committee E50 on Environmental Assessment, Risk Management and Corrective Action and is the direct responsibility of Subcommittee E50.47 on Biological Effects and Environmental Fate. Current edition approved Nov. 1, 2022. Published November 2022. Originally approved in 1991. Last previous edition approved in 2012 as E1415 – 91 (2012), which was withdrawn January 2021 and reinstated in November 2022. DOI: 10.1520/E1415-22. Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. 1 2. Referenced Documents

ASTM E1415-22 Referenced Document

• ASTM E1023 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
• ASTM E1192 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
• ASTM E1218 Standard Guide for Conducting Static 96-h Toxicity Tests with Microalgae
• ASTM E729 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
• ASTM E943 Standard Terminology Relating to Biological Effects and Environmental Fate*， 2023-06-01 Update
• IEEE/ASTM SI 10 American National Standard for Metric Practice

ASTM E1415-22 history

• 2022 ASTM E1415-22 Standard Guide for Conducting Static Toxicity Tests With Lemna gibba G3
• 2012 ASTM E1415-91(2012) Standard Guide for Conducting Static Toxicity Tests With Lemna gibba G3 (Withdrawn 2021)
• 1991 ASTM E1415-91(2004)e1 Standard Guide for Conducting Static Toxicity Tests With Lemna gibba G3
• 2004 ASTM E1415-91(2004) Standard Guide for Conducting Static Toxicity Tests With Lemna gibba G3
• 1991 ASTM E1415-91(1998) Standard Guide for Conducting Static Toxicity Tests With Lemna gibba G3