ASTM E1193-20
Standard Guide for Conducting Daphnia magna Life-Cycle Toxicity Tests

Standard No.

ASTM E1193-20

Release Date

2020

Published By

American Society for Testing and Materials (ASTM)

Latest

ASTM E1193-20

Scope

1.1 This guide covers procedures for obtaining laboratory data concerning the adverse effects of a test material (added to dilution water, but not to food) on Daphnia magna Straus, 1820, during continuous exposure throughout a life-cycle using the renewal or flow-through techniques. These procedures also should be useful for conducting life-cycle toxicity tests with other invertebrate species and cladocerans from the same genus (for example, Daphnia pulex), although modifications might be necessary. 1.2 These procedures are applicable to most chemicals, either individually or in formulations, commercial products, or known mixtures. With appropriate modifications, these procedures can be used to conduct tests on temperature, dissolved oxygen, pH, and on such materials as aqueous effluents (also see Guide E1192), leachates, oils, particulate matter, sediments, and surface waters. The technique, (renewal or flow-through), will be selected based on the chemical characteristics of the test material such as high oxygen demand, volatility, susceptibility to transformation (biologically or chemically), or sorption to glass. 1.3 Modification of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of standard test procedures. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information on new concepts and procedures for conducting life-cycle toxicity tests with D. magna. Appendix X3 provides modifications for conducting the chronic toxicity test method with D. pulex Leydig, 1860. 1.4 This guide is arranged as follows: Section Referenced Documents 2 Terminology 3 Summary of Guide 4 Significance and Use 5 Apparatus 6 Facilities 6.1 Construction Materials 6.2 Test Chambers 6.3 Cleaning 6.4 Acceptability 6.5 Reagents 7 Purity of Reagents 7.1 Hazards 8 Dilution Water 9 Requirements 9.1 Source 9.2 Treatment 9.3 Characterization 9.4 Test Material 10 General 10.1 Stock Solutions 10.2 Test Concentrations(s) 10.3 Test Organisms 11 Species 11.1 Age 11.2 Source 11.3 Brood Stock 11.4 Food 11.5 Handling 11.6 Harvesting Young 11.7 Quality 11.8 Procedure 12 Experimental Design 12.1 Dissolved Oxygen 12.2 Temperature 12.3 Loading 12.4 Selection of Test System 12.5 Beginning the Test 12.6 Care and Maintenance 12.7 Feeding 12.8 Duration 12.9 Biological Data 12.10 Other Measurements 12.11 Analytical Methodology 13 Acceptability of Test 14 Calculation of Results 15 Report 16 Keywords 17 Appendixes Appendix X1 Statistical Guidance Appendix X2 Food Appendix X3 Modifications for Conducting Chronic Life Cycle Analysis Tests with Daphnia Pulex 1 This guide is under the jurisdiction of ASTM Committee E50 on Environmental Assessment, Risk Management and Corrective Action and is the direct responsibility of Subcommittee E50.47 on Biological Effects and Environmental Fate. Current edition approved Dec. 1, 2020. Published January 2021. Originally approved in 1987. Last previous edition approved in 2012 as E1193 – 97 (2012). DOI: 10.1520/E1193-20. Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. 1 1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 8. 1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ASTM E1193-20 Referenced Document

• ASTM E1023 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
• ASTM E1192 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
• ASTM E1733 Standard Guide for Use of Lighting in Laboratory Testing
• ASTM E1847 Standard Practice for Statistical Analysis of Toxicity Tests Conducted Under ASTM Guidelines
• ASTM E729 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
• ASTM E943 Standard Terminology Relating to Biological Effects and Environmental Fate*， 2023-06-01 Update
• IEEE/ASTM SI 10 American National Standard for Metric Practice

ASTM E1193-20 history

• 2020 ASTM E1193-20 Standard Guide for Conducting Daphnia magna Life-Cycle Toxicity Tests
• 1997 ASTM E1193-97(2012) Standard Guide for Conducting Daphnia magna Life-Cycle Toxicity Tests
• 1997 ASTM E1193-97(2004) Standard Guide for Conducting Daphnia magna Life-Cycle Toxicity Tests
• 1997 ASTM E1193-97 Standard Guide for Conducting Daphnia magna Life-Cycle Toxicity Tests