ASTM D7687-17
Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration

Standard No.

ASTM D7687-17

Release Date

2017

Published By

American Society for Testing and Materials (ASTM)

Status

Be replaced

Replace By

ASTM D7687-21

Latest

ASTM D7687-23

Scope

1.1 This test method covers a protocol for capturing, extracting and quantifying the cellular adenosine triphosphate (cellular-ATP) content associated with microorganisms found in fuels and fuel-associated water. 1.2 The ATP is measured using a bioluminescence enzyme assay, whereby light is generated in amounts proportional to the concentration of cellular-ATP in the samples. The light is produced and measured quantitatively as relative light units (RLU) which are converted by comparison with an ATP standard, computation to pg ATP/mL and optional further transformation to Log10[pg ATP/mL]. 1.3 This test method is equally suitable for use as a laboratory or portable method. 1.4 This test method is limited to fuels with a nominal viscosity ≤75 cSt at test temperature. 1.5 This test method detects ATP concentrations in the range of 5.0 pg ATP/mL (≈0.699 log10[pg ATP/mL]) to 100 000 pg ATP/mL (≈5.000 log10[pg ATP/mL]) for 20 mL samples of fuel and 20 pg ATP/mL (≈1.301 log10[pg ATP/mL]) to 400 000 pg ATP/mL (≈5.602 log10[pg ATP/mL]) for 5 mL samples of fuel-associated water. NOTE 1—These ranges were calculated with the formula for calculating sample ATP in pg/mL provided in 12.1 based on the minimum recommended RLU for a 1 ng ⁄mL ATP standard when using the reagents specified in Section 7 and the luminometer specified in 6.4 and corrected with a reagent-method blank as determined in Appendix X5. 1.6 Providing interferences can be overcome, bioluminescence is a reliable and proven method for qualifying and quantifying ATP. This test method does not differentiate between ATP from different sources, for example: from different types of microorganisms, such as bacteria and fungi. 1.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. 1.9 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ASTM D7687-17 Referenced Document

• ASTM D1129 Standard Terminology Relating to Water
• ASTM D1655 Standard Specification for Aviation Turbine Fuels
• ASTM D2069 
• ASTM D2880 Standard Specification for Gas Turbine Fuel Oils
• ASTM D3699 Standard Specification for Kerosine
• ASTM D396 Standard Specification for Fuel Oils
• ASTM D4012 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
• ASTM D4175 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants*， 2023-07-01 Update
• ASTM D6161 Standard Terminology Used for Crossflow Microfiltration, Ultrafiltration, Nanofiltration, and Reverse Osmosis Membrane Processes
• ASTM D6300 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
• ASTM D6751 Standard Specification for Biodiesel Fuel Blendstock (B100) for Middle Distillate Fuels*， 2023-04-01 Update
• ASTM D7463 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water*， 2021-04-01 Update
• ASTM D7464 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing*， 2020-05-01 Update
• ASTM D7467 Standard Specification for Diesel Fuel Oil, Biodiesel Blend (B6 to B20)*， 2023-10-01 Update
• ASTM D7847 Standard Guide for Interlaboratory Studies for Microbiological Test Methods*， 2022-07-01 Update
• ASTM D7978 Standard Test Method for Determination of the Viable Aerobic Microbial Content of Fuels and Associated Water—Thixotropic Gel Culture Method*， 2019-07-01 Update
• ASTM D975 Standard Specification for Diesel Fuel Oils
• ASTM E2523 Standard Terminology for Metalworking Fluids and Operations*， 2023-11-01 Update
• ASTM E2694 Standard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids*， 2021-11-01 Update
• ASTM F1671 Standard Test Method for Resistance of Materials Used in Protective Clothing to Penetration by Blood-Borne Pathogens Using Phi-X174 Bacteriophage Penetration as a Test System

ASTM D7687-17 history

• 2023 ASTM D7687-23 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration
• 2021 ASTM D7687-21 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration
• 2017 ASTM D7687-17 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration
• 2011 ASTM D7687-11 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel, Fuel/Water Mixtures, and Fuel-Associated Water with Sample Concentration by Filtration