ASTM E2694-21
Standard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids

Standard No.

ASTM E2694-21

Release Date

2021

Published By

American Society for Testing and Materials (ASTM)

Latest

ASTM E2694-21

Scope

1.1 This test method provides a protocol for capturing, extracting, and quantifying the adenosine triphosphate (ATP) content associated with microorganisms found in watermiscible metalworking fluids (MWFs). 1.2 The ATP is measured using a bioluminescence enzyme assay, whereby light is generated in amounts proportional to the concentration of ATP in the samples. The light is produced and measured quantitatively as relative light units (RLUs) which are converted by comparison with an ATP standard and computation to pg ATP/mL. 1.3 This test method is equally suitable for use in the laboratory or field. 1.4 The test method detects ATP concentrations in the range of 4.0 pg ATP/mL to 400 000 pg ATP/mL. 1.5 Providing interferences can be overcome, bioluminescence is a reliable and proven method for qualifying and quantifying ATP. The method does not differentiate between ATP from different sources, for example, from different types of microorganisms, such as bacteria and fungi. 1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ASTM E2694-21 Referenced Document

• ASTM D1129 Standard Terminology Relating to Water
• ASTM D4012 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
• ASTM D4840 Standard Guide for Sample Chain-of-Custody Procedures
• ASTM D6161 Standard Terminology Used for Crossflow Microfiltration, Ultrafiltration, Nanofiltration, and Reverse Osmosis Membrane Processes
• ASTM E1326 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
• ASTM E1497 Standard Practice for Selection and Safe Use of Water-Miscible and Straight Oil Metal Removal Fluids*， 2023-04-01 Update
• ASTM E177 Standard Practice for Use of the Terms Precision and Bias in ASTM Test Methods
• ASTM E2523 Standard Terminology for Metalworking Fluids and Operations*， 2023-11-01 Update
• ASTM E691 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method

ASTM E2694-21 history

• 2021 ASTM E2694-21 Standard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids
• 2016 ASTM E2694-16 Standard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids
• 2011 ASTM E2694-11 Standard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids
• 2009 ASTM E2694-09 Standard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids