ASTM E1295-22
Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia

Standard No.

ASTM E1295-22

Release Date

2022

Published By

American Society for Testing and Materials (ASTM)

Latest

ASTM E1295-22

Scope

1.1 This guide describes procedures for obtaining data concerning the adverse effects of an effluent or a test material (added to dilution water, but not to food) on Ceriodaphnia dubia Richard 1894, during continuous exposure throughout a portion of the organism’s life. These procedures should also be useful for conducting life cycle toxicity tests with other Cladocera (Guide E1193), although modifications will be necessary. 1.2 These procedures are applicable to most chemicals, either individually or in formulations, commercial products, or known mixtures, that can be measured accurately at the necessary concentrations in water. With appropriate modifications these procedures can be used to conduct tests on temperature, dissolved oxygen, pH, dissolved ions, and on such materials as aqueous effluents (see also Guide E1192), leachates, oils, particulate matter, sediments (see also Guide E1706), and surface waters. Renewal tests might not be applicable to materials that have high oxygen demand, are highly volatile, are rapidly biologically or chemically transformed, or sorb to test chambers. If the concentration of dissolved oxygen falls below 4 mg/L or the concentration of test material decreases by more than 20 % in test solution(s) at any concentration between renewals, more frequent renewals might be necessary. 1.3 Other modifications of these procedures might be justified by special needs or circumstances. Results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparisons of results obtained using modified and unmodified versions of these procedures might provide useful information on new concepts and procedures for conducting three-brood toxicity tests with C. dubia. 1.4 This guide is arranged as follows: Section Referenced Documents 2 Terminology 3 Summary of Guide 4 Significance and Use 5 Apparatus 6 Facilities 6.1 Construction Materials 6.2 Test Chambers 6.3 Cleaning 6.4 Reagents and Materials 7 Hazards 8 Dilution Water 9 Requirements 9.1 Source 9.2 Treatment 9.3 Characterization 9.4 Test Material 10 General 10.1 Stock Solution 10.2 Effluent 10.3 Test Concentration(s) 10.4 Collection 10.5 Sample Containers 10.6 Preservation 10.7 Treatment 10.8 Test Organisms 11 Species 11.1 Age 11.2 Source 11.3 Brood Stock 11.4 Food 11.5 Handling 11.6 Quality 11.7 Procedure 12 Demonstration of Feasibility 12.1 Experimental Design 12.2 Dissolved Oxygen 12.3 Temperature 12.4 Preparing Test Solutions 12.5 Conditioning Test Chambers 12.6 Beginning a Test 12.7 Renewing Test Solutions 12.8 Duration of Test 12.9 Biological Data 12.10 Other Measurements 12.11 Test Material 12.12 Analytical Methodology 13 Acceptability of Test 14 Calculation 15 Report 16 Appendixes Food Appendix X1 Culture Techniques Appendix X2 Test Chambers Appendix X3 1 This guide is under the jurisdiction of ASTM Committee E50 on Environmental Assessment, Risk Management and Corrective Actionand is the direct responsibility of Subcommittee E50.47 on Biological Effects and Environmental Fate. Current edition approved Jan. 1, 2022. Published April 2022. Originally approved in 1989. Last previous edition approved in 2013 as E1295 – 01(2013). DOI: 10.1520/E1295-22. *A Summary of Changes section appears at the end of this standard Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. 1 Statistical Guidance Appendix X4 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 8. 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ASTM E1295-22 Referenced Document

• ASTM D1193 Standard Specification for Reagent Water
• ASTM D3978 Standard Practice for Algal Growth Potential Testing with Selenastrum Capricornutum
• ASTM D4447 Standard Guide for Disposal of Laboratory Chemicals and Samples
• ASTM E1023 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
• ASTM E1192 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
• ASTM E1193 Standard Guide for Conducting Daphnia magna Life-Cycle Toxicity Tests
• ASTM E1706 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
• ASTM E1733 Standard Guide for Use of Lighting in Laboratory Testing
• ASTM E1847 Standard Practice for Statistical Analysis of Toxicity Tests Conducted Under ASTM Guidelines
• ASTM E729 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
• ASTM E943 Standard Terminology Relating to Biological Effects and Environmental Fate*， 2023-06-01 Update

ASTM E1295-22 history

• 2022 ASTM E1295-22 Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia
• 2001 ASTM E1295-01(2013) Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia
• 2001 ASTM E1295-01(2006) Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia
• 2001 ASTM E1295-01 Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia
• 1989 ASTM E1295-89(1995) Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia