This International Standard specifies a method for determining the dehydrogenase activity of soils using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride (INT). Because INT reduction is less sensitive to O2, the method is more reproducible than the TTC method described in ISO 23753-1. This standard does not apply to the determination of dehydrogenase activity in support horizons (L, F, H) of forest humus forms with low microbial activity (e.g. bog) or in soils with reducing properties (e.g. waterlogged soils).
EN ISO 23753-2:2011 Referenced Document
ISO 10381-6:1993 Soil quality; sampling; part 6: guidance on the collection, handling and storage of soil for the assessment of aerobic microbial processes in the laboratory
ISO 11259:1998 Soil quality - Simplified soil description
ISO 11465:1993 Soil quality; determination of dry matter and water content on a mass basis; gravimetric method
EN ISO 23753-2:2011 history
2020EN ISO 23753-2:2019/A1:2020 Soil quality - Determination of dehydrogenases activity in soils - Part 2: Method using iodotetrazolium chloride (INT) - Amendment 1 (ISO 23753-2:2019/Amd 1:2020)
2019EN ISO 23753-2:2019 Soil quality - Determination of dehydrogenases activity in soils - Part 2: Method using iodotetrazolium chloride (INT)
2011EN ISO 23753-2:2011 Soil Quality - Determination of dehydrogenase activity in soils - Part 2: Method using iodotetrazolium chloride (INT)