ISO 16266-2:2018
Water quality - Detection and enumeration of Pseudomonas aeruginosa - Part 2: Most probable number method

Standard No.

ISO 16266-2:2018

Release Date

2018

Published By

International Organization for Standardization (ISO)

Latest

ISO 16266-2:2018

Scope

This document specifies a method for the enumeration of Pseudomonas aeruginosa in water. The method is based on the growth of target organisms in a liquid medium and calculation of the most probable number (MPN) of organisms by reference to MPN tables. This document is applicable to a range of types of water. For example, hospital waters, drinking water and non‐carbonated bottled waters intended for human consumption, groundwater, swimming pool and spa pool waters including those containing high background counts of heterotrophic bacteria. This document does not apply to carbonated bottled waters, flavoured bottle waters, cooling tower waters or marine waters, for which the method has not been validated. These waters are, therefore, outside the scope of this document. Laboratories can employ the method presented in this document for these matrices by undertaking appropriate validation of performance of this method prior to use. The test is based on a bacterial enzyme detection technology that signals the presence of P. aeruginosa through the hydrolysis of a 7‐amino‐4‐methylcoumarin aminopeptidase substrate present in a special reagent. P. aeruginosa cells rapidly grow and reproduce using the rich supply of amino acids, vitamins and other nutrients present in the reagent. Actively growing strains of P. aeruginosa have an enzyme that cleaves the 7‐amido‐coumarin aminopeptidase substrate releasing a product which fluoresces under ultraviolet (UV) light. The test described in this document provides a confirmed result within 24 h with no requirement for further confirmation of positive wells.

ISO 16266-2:2018 Referenced Document

• ISO 11133:2014 Microbiology of food, animal feed and water - Preparation, production, storage and performance testing of culture media
• ISO 19458:2006 Water quality - Sampling for microbiological analysis
• ISO 8199:2005 Water quality - General guidance on the enumeration of micro-organisms by culture
• ISO/IEC GUIDE 2:2004 Standardization and related activities - General vocabulary

ISO 16266-2:2018 history

• 2018 ISO 16266-2:2018 Water quality - Detection and enumeration of Pseudomonas aeruginosa - Part 2: Most probable number method