ASTM D7304-16
Standard Test Method for Determination of Denatonium Ion in Engine Coolant by HPLC
- Standard No.
- ASTM D7304-16
- Release Date
- 2016
- Published By
- American Society for Testing and Materials (ASTM)
- Status
- Replace By
- ASTM D7304-22
- Latest
- ASTM D7304-22a
- Scope
4.1 This test method provides for the qualitative and quantitative determination of denatonium benzoate in engine coolant in milligrams per litre to low percent range and requires approximately 1 mL per test, with results available in less than 10 min. Denatonium benzoate is a compound composed of a quaternary ammonium cation, denatonium and an inert anion, benzoate. In solution the denatonium benzoate exists in equilibrium between the denatonium benzoate compound, the denatonium cation and benzoate anion. By slightly adjusting the pH of the solution to be more acidic (≈ pH 4.6) the equilibrium will be shifted to the direction of forming more denatonium and benzoate ions in the solution.
1.1 This test method covers the chemical analysis of engine coolant for denatonium benzoate (DNB) by high-performance liquid chromatography (HPLC). DNB is added to potentially render engine coolant unpalatable to animals and humans. This analytical method was designed for the analysis of DNB and is not valid for any other bittering agents such as denatonium saccharide.
1.2 This test method is applicable to both new and used coolants.
1.3 Coelution of other ions may cause interferences in the detection of the denatonium cation. In the case of unfamiliar formulations, identification verification should be performed by either or both fortification and dilution of the sample matrix with denatonium ion.
1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
ASTM D7304-16 Referenced Document
- ASTM D1176 Standard Practice for Sampling and Preparing Aqueous Solutions of Engine Coolants or Antirusts for Testing Purposes
- ASTM D1193 Standard Specification for Reagent Water
- ASTM E177 Standard Practice for Use of the Terms Precision and Bias in ASTM Test Methods
- ASTM E691 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
ASTM D7304-16 history
- 2022 ASTM D7304-22a Standard Test Method for Determination of Denatonium Benzoate in Engine Coolant by HPLC
- 2022 ASTM D7304-22 Standard Test Method for Determination of Denatonium Benzoate in Engine Coolant by HPLC
- 2016 ASTM D7304-16 Standard Test Method for Determination of Denatonium Ion in Engine Coolant by HPLC
- 2014 ASTM D7304-14 Standard Test Method for Determination of Denatonium Ion in Engine Coolant by HPLC
- 2006 ASTM D7304-06 Standard Test Method for Determination of Denatonium Ion in Engine Coolant by HPLC
