This International Standard specifies the plant-based test, hereinafter referred to as biotest. It enables the determination of the environmentally relevant bioavailability of trace elements for plants, either fundamentally as the concentration in shoots and roots or more integratively as the net uptake flux in plants. Carrying out the bioassay involves two sequential steps: (i) pre-growth of plants in hydroponics and (ii) growth of plants in contact with soil samples. The concentration in the shoots and roots as well as the net uptake flux of trace elements in plants are determined at the end of the second step of the bioassay. This bioassay applies to the assessment of the environmentally relevant bioavailability of trace elements for plants, in particular cultivated plants, in soil or soil materials under oxic conditions, taking into account that in the standardized bioassay three plant species (cabbage, Brassica oleracea; tall fescue, Festuca arundinacea; tomato, Lycopersicon esculentum; 7.1) are recommended, but additional target plant species can also be used (see 7.1, Appendix A), and the standardized bioassay for a range of trace elements including arsenic (As), cadmium (Cd), chromium (Cr), cobalt (Co) , copper (Cu), lead (Pb), nickel (Ni) and zinc (Zn) is validated, but other trace elements can also be taken into account (see Appendix A). The bioassay can be applied to soil and soil materials, including soil, to which compost, sludge, wastewater and other (waste) materials have been added before or after on-site sampling. NOTE 1 This bioassay is not intended to assess the environmental bioavailability of trace elements that are prone to volatilization or result from ingestion such as: B. occurs following atmospheric precipitation in the plant leaves. NOTE 2 This bioassay is not intended for the assessment of environmental relevance
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EN ISO 16198:2015 history
2015EN ISO 16198:2015 Soil quality - Plant-based test to assess the environmental bioavailability of trace elements to plants