EN ISO 9308-1:2014 Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora (Incorporates Amendment A1: 2017)
This part of ISO 9308 describes a procedure for the payment of Escherichia coli (E. coli) and coliform bacteria. The process is based on membrane filtration, subsequent cultivation on chromogenic coliform agar (CCA) and calculation of the number of target organisms in the sample. Due to the low selectivity of the agar for primary enrichment, the accompanying flora can interfere with a reliable payment of E. coli and coliform bacteria, e.g. B. in surface water or water from wells near the surface. This procedure is not suitable for such types of water. This part of ISO 9308 is particularly suitable for testing waters with low bacterial counts, where fewer than 100 total colonies grow on Chromogenic Coliform Agar (CCA). This can be drinking water, disinfected swimming pool water or prepared water from water treatment plants. Some E. coli strains that are -D-glucuronidase negative, such as B. Escherichia coli O157, are not determined as E. coli. Because they are -D-galactosidase positive, they appear as coliform bacteria on a chromogenic medium agar.
EN ISO 9308-1:2014 Referenced Document
ISO 7218:2007 Microbiology of food and animal feeding stuffs - General requirements and guidance for microbiological examinations
EN ISO 9308-1:2014 history
2017EN ISO 9308-1:2014/A1:2017 Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora (ISO 9308-1:2014/Amd 1:2016)
2014EN ISO 9308-1:2014 Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora (Incorporates Amendment A1: 2017)
2000EN ISO 9308-1:2000 Water Quality - Detection and Enumeration of Escherichia Coli and Coliform Bacteria - Part 1: Membrane Filtration Method