ISO 16198:2015
Soil quality - Plant-based test to assess the environmental bioavailability of trace elements to plants

Standard No.

ISO 16198:2015

Release Date

2015

Published By

International Organization for Standardization (ISO)

Latest

ISO 16198:2015

Scope

This International Standard specifies the plant-based test, hereafter called the biotest. It enables estimation of the environmental bioavailability of trace elements to plants either basically as the concentration in shoots and roots or in a more integrative way as the net uptake flux in plants. The biotest procedure includes two successive steps: (i) a pre-growth of plants in hydroponics and (ii) a growth of plants in contact with soil samples. The concentration in shoots and roots as well as the net uptake flux of trace elements in plants are determined at the end of the second step of the biotest procedure. This biotest is applicable to the assessment of environmental bioavailability of trace elements to plants, more particularly to agricultural plants, in soils or soil materials under oxic conditions, considering that — three plant species (cabbage, Brassica oleracea; tall fescue, Festuca arundinacea; tomato, Lycopersicon esculentum; 7.1) are suggested in the standardized biotest procedure, but additional target-plant species can also be used (see 7.1, Annex A), and — the standardized biotest procedure is validated for a range of trace elements including arsenic (As), cadmium (Cd), chromium (Cr), cobalt (Co), copper (Cu), lead (Pb), nickel (Ni), and zinc (Zn), but additional trace elements can also be accounted for (see Annex A). The biotest can be applied to soils and soil materials, including soils amended before or after field sampling with composts, sludges, wastewaters, and other (waste) materials. NOTE 1 This biotest is not designed to assess the environmental bioavailability of trace elements that are prone to volatilisation or resulting from uptake occurring in plant leaves following, e.g. atmospheric fallout. NOTE 2 This biotest is not designed to assess the environmental bioavailability to plants of organic contaminants. A similar experimental procedure could be used but the physical separation between plant roots and soil using a polyamide mesh needs to be adapted to avoid organic contaminant sorption on the mesh.

ISO 16198:2015 Referenced Document

• ISO 10390:2005 Soil quality - Determination of pH
• ISO 10693:1995 Soil quality - Determination of carbonate content - Volumetric method
• ISO 10694:1995 Soil quality - Determination of organic and total carbon after dry combustion (elementary analysis)
• ISO 11047:1998 Soil quality - Determination of cadmium, chromium, cobalt, copper, lead, manganese, nickel and zinc in aqua regia extracts of soil - Flame and electrothermal atomic absorption spectrometric methods
• ISO 11074:2005 Soil quality - Vocabulary
• ISO 11269-1:2012 Soil quality - Determination of the effects of pollutants on soil flora - Part 1: Method for the measurement of inhibition of root growth
• ISO 11269-2:2012 Soil quality - Determination of the effects of pollutants on soil flora - Part 2: Effects of contaminated soil on the emergence and early growth of higher plants
• ISO 11277:2009 Soil quality - Determination of particle size distribution in mineral soil material - Method by sieving and sedimentation
• ISO 11465:1993 Soil quality; determination of dry matter and water content on a mass basis; gravimetric method
• ISO 13528:2005 Statistical methods for use in proficiency testing by interlaboratory comparisons
• ISO 14869-1:2001 Soil quality - Dissolution for the determination of total element content - Part 1: Dissolution with hydrofluoric and perchloric acids
• ISO 17126:2005 Soil quality - Determination of the effects of pollutants on soil flora - Screening test for emergence of lettuce seedlings (Lactuca sativa L.)
• ISO 17294-2:2003 Water quality - Application of inductively coupled plasma mass spectrometry (ICP-MS) - Part 2: Determination of 62 elements
• ISO 17402:2008 Soil quality - Requirements and guidance for the selection and application of methods for the assessment of bioavailability of contaminants in soil and soil materials
• ISO 197 Copper and copper alloys; Terms and definitions; Part 5 : Methods of processing and treatment
• ISO 3696:1987 Water for analytical laboratory use; Specification and test methods
• ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results - Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method

ISO 16198:2015 history

• 2015 ISO 16198:2015 Soil quality - Plant-based test to assess the environmental bioavailability of trace elements to plants