ASTM D5392-14
Standard Test Method for Isolation and Enumeration of Escherichia Coli in Water by the Two-Step Membrane Filter Procedure
- Standard No.
- ASTM D5392-14
- Release Date
- 2014
- Published By
- American Society for Testing and Materials (ASTM)
- Status
- Replace By
- ASTM D5392-19
- Latest
- ASTM D5392-24
- Scope
5.1 This test method is useful for measuring recreational water quality and chlorinated wastewaters, although it can be used for any water suspected of contamination by fecal wastes of warm-blooded animals. The significance of finding E. coli in recreational water samples, especially samples obtained from fresh recreational waters, is that there is a risk of gastrointestinal illness, directly related to the E. coli density, associated with swimming.5
5.2 Since small or large volumes of water or dilutions thereof can be analyzed by the MF technique, a wider range of levels of E. coli in water can be detected and enumerated than with other methods.
1.1 This test method describes a membrane filter (MF) procedure for the detection and enumeration of Escherichia coli, a bacterium found exclusively in the feces of humans and other warm-blooded animals. The presence of these microorganisms in water is an indication of fecal pollution and the possible presence of enteric pathogens. These bacteria are found in water and wastewater in a wide range of densities. The detection limit of this procedure is one colony forming unit (CFU) per volume filtered.
1.2 This test method has been used successfully with temperate fresh and marine ambient waters, and wastewaters. It is the user's responsibility to ensure the validity of this test method for waters of other types.
1.3 The values stated in SI units are to be regarded as standard.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific hazard statements, see Section 9.
ASTM D5392-14 Referenced Document
- ASTM D1129 Standard Terminology Relating to Water
- ASTM D1193 Standard Specification for Reagent Water
- ASTM D3370 Standard Practices for Sampling Water from Closed Conduits
- ASTM D3870 Standard Practice for Establishing Performance Characteristics for Colony Counting Methods in Microbiology
- ASTM D5465 Standard Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
ASTM D5392-14 history
- 2024 ASTM D5392-24 Standard Test Method for Isolation and Enumeration of
Escherichia coli in Water by the Two-Step Membrane Filter Procedure - 2019 ASTM D5392-19 Standard Test Method for Isolation and Enumeration of
Escherichia coli in Water by the Two-Step Membrane Filter Procedure - 2014 ASTM D5392-14 Standard Test Method for Isolation and Enumeration of
Escherichia Coli in Water by the Two-Step Membrane Filter Procedure - 1993 ASTM D5392-93(2006) Standard Test Method for Isolation and Enumeration ofEscherichia Coli in Water by the Two-Step Membrane Filter Procedure
- 2000 ASTM D5392-93(2000) Standard Test Method for Isolation and Enumeration of Escherichia Coli in Water by the Two-Step Membrane Filter Procedure
