VDI 4331 Blatt 2-2013
Monitoring the effects of genetically modified organisms (GMOs) - Method for extracting nucleic acids from soil for the analysis of microbial communities and detection of transgenic DNA - Quality requirements and applications

Standard No.

VDI 4331 Blatt 2-2013

Release Date

2013

Published By

Association of German Mechanical Engineers

Latest

VDI 4331 Blatt 2-2013

Replace

VDI 4331 Blatt 7-2011

Scope

Extracted nucleic acids from soil can provide the source material for detecting microorganisms living in soil and can help determining their diversity. Soil nucleic acids can also be used to detect plant genes, for example transgenic DNA (recombinant genes) from genetically modified plants. The standard describes the basic principles and gives sample protocols for the extraction of nucleic acids from soil. This standard describes the quality requirements for the analysis of agricultural soils in order to determine the effects of the release or cultivation of genetically modified organisms and the associated changes in land use on the structure and function

VDI 4331 Blatt 2-2013 Referenced Document

• DIN EN ISO 21571:2005 Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Nucleic acid extraction
• DIN EN ISO 24276:2006 Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - General requirements and definitions
• DIN ISO 10381-1:2003 Soil quality - Sampling - Part 1: Guidance on the design of sampling programmes (ISO 10381-1:2002)
• DIN ISO 10381-6:2009 Soil quality - Sampling - Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory (ISO 10381-6:2009);English version of DIN ISO 1038
• VDI 1000-2010 VDI Guideline Work - Principles and procedures
• VDI 4330 Blatt 1-2006 Monitoring the ecological effects of genetically modified organisms - Genetically modified plants - Basic principles and strategies
• VDI 4330 Blatt 7-2006 Monitoring the effects of genetically modified organisms (GMO) - Qualitative methods for the detection of genetically engineered nucleic acids in the environment
• VDI 4331 Blatt 1-2013 Monitoring der Wirkung von gentechnisch veraenderten Organismen (GVO) - Wirkung auf Bodenorganismen

VDI 4331 Blatt 2-2013 history

• 2013 VDI 4331 Blatt 2-2013 Monitoring the effects of genetically modified organisms (GMOs) - Method for extracting nucleic acids from soil for the analysis of microbial communities and detection of transgenic DNA - Quality requirements and applications
• 2011 VDI 4331 Blatt 7-2011 Monitoring der Wirkungen gentechnisch veraenderter Organismen (GVO) - Verfahren zur Extraktion von Nukleinsaeuren aus Boeden zur Analyse von mikrobiellen Gemeinschaften und Nachweis transgener DNA - Qualitaetsanforderungen und Anwendungsbeispiele

VDI 4331 Blatt 2-2013 Monitoring the effects of genetically modified organisms (GMOs) - Method for extracting nucleic acids from soil for the analysis of microbial communities and detection of transgenic DNA - Quality requirements and applications has been changed from VDI 4331 Blatt 7-2011 Monitoring der Wirkungen gentechnisch veraenderter Organismen (GVO) - Verfahren zur Extraktion von Nukleinsaeuren aus Boeden zur Analyse von mikrobiellen Gemeinschaften und Nachweis transgener DNA - Qualitaetsanforderungen und Anwendungsbeispiele.