This European Standard describes a method for the detection of those serotypes of enterovirus that replicate and form plaques in BGM cells under agar. These will largely be poliovirus and oxsackievirus B serotypes. Most serotypes of echovirus, Coxsackievirus A and animal enteroviruses are unlikely to produce plaques under these conditions. Reoviruses are unlikely to be detectable within the specified seven day incubation period. It is applicable to any type of water and processed water sample although toxic elements may interfere with cell culture. The methods to confirm that the plaques seen contain enterovirus are given. Methods to identify which serotype
DS/EN 14486:2006 history
2006DS/EN 14486:2006 Water quality - Detection of human enteroviruses by monolayer plaque assay