DS/EN ISO 12966-3:2010
Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 3: Preparation of methyl esters using trimethylsulfonium hydroxide (TMSH)

Standard No.

DS/EN ISO 12966-3:2010

Release Date

2010

Published By

Danish Standards Foundation

Latest

DS/EN ISO 12966-3:2010

Scope

This part of ISO 12966 specifies a rapid base-catalysed transesterification method of fats and oils withtrimethylsulfonium hydroxide (TMSH) to prepare fatty acid methyl esters. The method is exclusively applicable to the preparation of methyl esters of fats and oils for gas liquid chromatographic (GLC) analysis. It is applicable to all fats and oils including milk fat and blends containing milk fat. Isomerization of unsaturated fatty acids only occurs to a minor extent and isomerized fatty acids are only present at the determination limit.As isomerization takes place, the procedure is not recommended for conjugated linoleic acid (CLA). As CLA is not correct

DS/EN ISO 12966-3:2010 history

• 2010 DS/EN ISO 12966-3:2010 Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 3: Preparation of methyl esters using trimethylsulfonium hydroxide (TMSH)