ISO 16014-2:2012
Plastics - Determination of average molecular mass and molecular mass distribution of polymers using size-exclusion chromatography - Part 2: Universal calibration method
- Standard No.
- ISO 16014-2:2012
- Release Date
- 2012
- Published By
- International Organization for Standardization (ISO)
- Status
- Replace By
- ISO 16014-2:2019
- Latest
- ISO 16014-2:2019
- Scope
- This part of ISO 16014 specifies a method for determining the average molecular mass and the molecular mass distribution of polymers using size-exclusion chromatography (SEC). The average molecular mass and the molecular mass distribution are calculated using a universal calibration curve instead of the conventional calibration curve. A list of documents related to this part of ISO 16014 is given in the Bibliography. NOTE The concept of the universal calibration method is based on a relationship by which the retention time in SEC depends on the size of the polymer molecule (the hydrodynamic volume) or on the product of the intrinsic viscosity [η] and the molecular mass M. Many polymers classified as random-coil polymers (regardless of their chemical structure, degree of branching, composition or tacticity) have been experimentally confirmed as following this relationship. The universal calibration curve is prepared by plotting the logarithm of [η]M against the elution time t or elution volume V using polymer standards such as polystyrene with narrow molecular mass distributions. The molecular mass Mi of an unknown polymer sample can then be calculated from the value of [η]M at each retention time using the universal calibration curve and [η] or K and a in the Mark-Houwink-Sakurada equation ([η] = KMa → [η]M = KMa+1), where K is a constant and a is an exponent depending on the experimental conditions. Therefore this test method is classified as a relative method as described in or nearly equal to, the absolute values.
ISO 16014-2:2012 Referenced Document
- ISO 16014-1:2012 Plastics - Determination of average molecular mass and molecular mass distribution of polymers using size-exclusion chromatography - Part 1: General principles
- ISO 16014-3:2012 Plastics - Determination of average molecular mass and molecular mass distribution of polymers using size-exclusion chromatography - Part 3: Low-temperature method
- ISO 16014-4:2012 Plastics - Determination of average molecular mass and molecular mass distribution of polymers using size-exclusion chromatography - Part 4: High-temperature method
- ISO 472:1999 Plastics - Vocabulary
ISO 16014-2:2012 history
- 2019 ISO 16014-2:2019 Plastics — Determination of average molecular weight and molecular weight distribution of polymers using size-exclusion chromatography — Part 2: Universal calibration method
- 2012 ISO 16014-2:2012 Plastics - Determination of average molecular mass and molecular mass distribution of polymers using size-exclusion chromatography - Part 2: Universal calibration method
- 2003 ISO 16014-2:2003 Plastics - Determination of average molecular mass and molecular mass distribution of polymers using size-exclusion chromatography - Part 2: Universal calibration method
