This standard specifies the HPLC method for the determination of butyl hydroxyanisole (BHA), dibutyl hydroxytoluene (BHT), ethoxyquin (EQ) and propyl gallate (PG) in feed and the determination method of butyl in feed. The gas chromatography determination method of hydroxyanisole (BHA), butylated hydroxytoluene (BHT), ethoxyquin (EQ). This standard high performance liquid chromatography method is suitable for the determination of butylated hydroxyanisole (BHA), dibutyl hydroxytoluene (BHT), ethoxyquin (EQ) and Determination of propyl gallate (PG) content; gas chromatography determination method is suitable for the determination of butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and ethoxyquin (EQ) content in compound feed and fish meal . The detection limit of the HPLC determination method is: butylated hydroxyanisole (BHA) 1.1 mg/kg, butylated hydroxytoluene (BHT) 1.6 mg/kg, ethoxyquin (EQ) 1.8 mg/kg , propyl gallate (PG) 1.3 mg/kg; limit of quantification: butylated hydroxyanisole (BHA) 5.6 mg/kg, dibutyl hydroxytoluene (BHT) 8.0 mg/kg, ethoxyquin (EQ) 8.8 mg/kg, propyl gallate (PG) 6.5 mg/kg.