This part of ISO 23753 specifies a method for determining soil dehydrogenase activity using
2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride (INT). As the INT reduction is less sensitive to
O2, the method is more reproducible than the TTC-method described in ISO 23753-1.
It is not applicable for determining the dehydrogenase activity in upper layers (L, F, H horizons) of forest
humus forms with low microbial activity (e.g. mor), or in soils showing reducing properties (e.g. waterlogged
soils).
BS EN ISO 23753-2:2011 Referenced Document
ISO 10381-6 Soil quality - Sampling - Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
ISO 10390 Soil, treated biowaste and sludge - Determination of pH*， 2021-03-31 Update
ISO 11259 Soil quality - Simplified soil description
ISO 11465 Soil quality; determination of dry matter and water content on a mass basis; gravimetric method; technical corrigendum 1
BS EN ISO 23753-2:2011 history
2021BS EN ISO 23753-2:2019+A1:2020 Soil quality Determination of dehydrogenases activity in soils - Method using iodotetrazolium chloride (INT)
2019BS EN ISO 23753-2:2019 Soil quality. Determination of dehydrogenases activity in soils-Method using iodotetrazolium chloride (INT)
2011BS EN ISO 23753-2:2011 Soil quality. Determination of dehydrogenase activity in soils. Method using iodotetrazolium chloride (INT)