This International Standard gives general guidelines for enumeration of yeast and mould present in cosmetics
by counting the colonies on selective agar medium after aerobic incubation.
In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate
microbiological risk analysis so as to determine the types of cosmetic products to which this International
Standard is applicable. Products considered to present a low microbiological risk include those with low water
activity, hydro-alcoholic products, products with extreme pH values, etc.
Because of the large variety of cosmetic products within this field of application, this method might not be
suited to some products in every detail (e.g. certain water-immiscible products). Other methods (e.g.
automated) can be used for the test presented here provided that their equivalence has been demonstrated or
the method has been otherwise validated.
Yeast enumerated can be identified using suitable identification tests, for example tests described in the
standards listed in the Bibliography. Mould enumerated can be identified by other appropriate methods, if
necessary.
BS EN ISO 16212:2011 Referenced Document
EN 12353 Chemical disinfectants and antiseptics - Preservation of test organisms used for the determination of bactericidal (including Legionella), mycobactericidal, sporicidal, fungicidal and virucidal (including bacteriophages) activity*， 2021-08-31 Update
ISO 21148 Cosmetics - Microbiology - General instructions for microbiological examination*， 2017-06-01 Update