Warning—Personnel using this standard should have practical experience in regular laboratory work. This standard does not address all possible safety is ues. Users are responsible for taking appropriate safety and health measures and ensuring compliance with the conditions stipulated in relevant national regulations. This standard specifies the molecular biology detection methods of Cucumber mosaic virus (CMV) double-antibody sandwich enzyme-linked immunosorbent assay (DAS ELis ) and reverse transcription polymerase chain reaction (RT-PCR) in pepper seedlings. This standard is applicable to the detection of cucumber mosaic virus in pepper cuttings and cladodes of cuttings.