This Technical Specification (CEN/TS 15606:2009) specifies a high performance liquid chromatographic (HPLC) method with UV-detection for the determination of acesulfame-K, aspartame, neohesperidine- dihydrochalcone and saccharin in foodstuffs. The method has been fully validated [1] for the dialysis procedure through collaborative trial (see 8.2, 8.3 and Annex C), according to the IUPAC Harmonised Protocol [2], on the following analyte matrix combinations: acesulfame-K (from 86 mg/l to 331 mg/l) and aspartame (from 97 mg/kg to 610 mg/l)in water-based drink, fruit-based drink, cheesecake with biscuit base, canned soup and instant chocolate drink saccharin (from 70 mg/l to 97 mg/kg) in water-based drink, fruit-based drink, cheesecake with biscuit base and canned soup; neohesperidine-dihydrochalcone (from 27 mg/l to 57 mg/kg)in water-based drink, fruit-based drink and canned soup.
PD CEN/TS 15606:2009 Referenced Document
EN ISO 3696:1995 Water for Analytical Laboratory Use- Specification and Test Methods (ISO 3696 : 1987)
PD CEN/TS 15606:2009 history
2009PD CEN/TS 15606:2009 Foodstuffs - Determination of acesulfame-K, aspartame, neohesperidine-dihydrochalcone and saccharin - High performance liquid chromatographic method