DIN EN 12393-2:2009
Foods of plant origin - Multiresidue methods for the gas chromatographic determination of pesticide residues - Part 2: Methods for extraction and cleanup; English version of DIN EN 12393-2:2009-01

Standard No.

DIN EN 12393-2:2009

Release Date

2009

Published By

German Institute for Standardization

Status

Withdraw

Replace By

DIN EN 12393-2:2014

DIN EN 12393-2 E:2012-03

Latest

DIN EN 12393-2:2014-03

Scope

This European Standard specifies methods for the extraction and clean-up of food samples of plant origin for quantitative determination of pesticide residues. Different solvents can be used for this purpose. These pesticide residues are generally associated with other co-extracted compounds which would interfere in the analysis. To purify the crude extracts to be analysed, several techniques can be used. This standard contains the following extraction and clean-up methods that have been subjected to interlaboratory studies and/or are adopted throughout Europe: -- method L: Extraction with acetone, liquid-liquid partition with dichloromethane and clean-up on a silica gel/charcoal column [1]; -- method M: Extraction with acetone and liquid-liquid partition with dichloromethane/light petroleum, if necessary clean-up on Florisil 1 ) [2], [3], [4]; -- method N: Extraction with acetone, liquid-liquid partition with dichloromethane or cyclohexane/ethyl acetate and clean-up with gel permeation and silica gel chromatography [5], [6]; -- method P: Extraction with ethyl acetate, and if necessary, clean-up by gel permeation chromatography [7]. This European Standard specifies the details of methods L to P for the extraction and the clean-up of food samples of plant origin. Several solvents at different volumes are used for extraction. Techniques of clean-up are listed such as liquid-liquid partition, liquid chromatography on various adsorbents and gel permeation chromatography. A table providing the couples (matrix/pesticide) which have been submitted to collaborative studies and a list of indicative applicability of the method to different pesticides are given for each method, wherever possible.

DIN EN 12393-2:2009 history

• 2014 DIN EN 12393-2:2014-03 Foods of plant origin - Multiresidue methods for the determination of pesticide residues by GC or LC-MS/MS - Part 2: Methods for extraction and cleanup; German version EN 12393-2:2013
• 2014 DIN EN 12393-2:2014 Foods of plant origin - Multiresidue methods for the determination of pesticide residues by GC or LC-MS/MS - Part 2: Methods for extraction and cleanup; German version EN 12393-2:2013
• 2009 DIN EN 12393-2:2009 Foods of plant origin - Multiresidue methods for the gas chromatographic determination of pesticide residues - Part 2: Methods for extraction and cleanup; English version of DIN EN 12393-2:2009-01