International Organization for Standardization (ISO)
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ISO 27368:2008
Scope
This International Standard details analytical methods suitable for analysing the two primary toxic combustion
gases, carbon monoxide (CO) and hydrogen cyanide (HCN), in blood samples collected from fire casualties.
In blood, CO is measured as carboxyhaemoglobin (COHb) and HCN as cyanide ion (CN-). Although
numerous methods are reported in the literature for performing blood COHb and CN- analyses, the analytical
methods included herein are based upon their suitability for performing the analysis on ante-mortem and post-
mortem blood samples from fire casualties. The analytical principle, analysis time, repeatability, reproducibility,
robustness, effectiveness and instruments used are considered for those methods. Some of the methods
described herein might not be suitable for analysing putrid or clotted blood. Burned (solid) blood can be
analysed after homogenization.
ISO 27368:2008 Referenced Document
ISO 13344 Estimation of the lethal toxic potency of fire effluents*, 2015-12-01 Update
ISO 13943 Fire safety — Vocabulary*, 2023-09-01 Update
ISO 19701 Methods for sampling and analysis of fire effluents*, 2013-04-01 Update
ISO 3696:1987 Water for analytical laboratory use; Specification and test methods
ISO/TS 13571 Life-threatening components of fire - Guidelines for the estimation of time available for escape using fire data
ISO 27368:2008 history
2008ISO 27368:2008 Analysis of blood for asphyxiant toxicants - Carbon monoxide and hydrogen cyanide