ASTM E1279-89(2008)
Standard Test Method for Biodegradation By a Shake-Flask Die-Away Method

Standard No.

ASTM E1279-89(2008)

Release Date

1989

Published By

American Society for Testing and Materials (ASTM)

Status

Withdraw 2013-03

Latest

ASTM E1279-89(2008)

Scope

Most of the simpler methods used to screen chemicals for biodegradation potential employ measurements that are not specific to the test substance, such as loss of dissolved organic carbon, evolution of respiratory carbon dioxide, or uptake of dissolved oxygen. Such methods generally are used to evaluate the transformation of the test substance to carbon dioxide, water, oxides or mineral salts of other elements, or products associated with the normal metabolic processes of microorganisms (ultimate biodegradability), or both. These methods require the use of relatively high initial concentrations of the test substance, generally 10 mg/L or higher, unless the tests are conducted using ¹⁴C-radiolabeled test compounds. Biodegradation tests measuring ¹⁴C-CO₂ evolution, for example, can be conducted using initial concentration of test compound at parts per billion. These tests, however, require specialized equipment and the custom preparation of appropriately labeled compound is often very expensive.

Die-away biodegradation methods are simple simulation methods that employ water collected from natural water sources and follow the disappearance of an added amount of the test substance resulting from the activity of microorganisms in the water sample. The chemical-specific analytical techniques used to follow the disappearance of the test substance, typically are employed using relatively low initial concentrations of the test substance. Most environmental pollutants are present in the environment at relatively low concentrations (less than 1 mg/L) and it has been observed that biodegradation rates obtained using high test compound concentrations may be quite different from those observed at lower concentrations (6).

The transformation of the test substance to an extent sufficient to remove some characteristic property of the molecule, resulting in the loss of detection by the chemicalspecific analytical technique, is referred to as primary biodegradation. For many purposes, evidence of primary biodegradation is sufficient, especially when it is known or can be shown that toxicity, or some other undesirable feature, associated with the test compound is removed or significantly reduced as a result of the primary biodegradation. A determination of ultimate biodegradation, on the other hand, is usually required only when treatability or organic loading are issues of concern. Furthermore, many of the simpler methods, such as those measuring CO₂ evolution (see 4.1), may not detect primary biodegradation.

The use of low substrate concentration enhances the probability of observing first-order, or pseudo first-order, kinetics. Thus, a rate constant for the primary biodegradation reaction and a half-life can be derived from the test compound under defined incubation conditions. Rate constants are required in many environmental fate mathematical models.

1.1 This test method describes procedures for assessing the biodegradation of chemicals in natural surface water samples.

1.2 This test method provides an opportunity to evaluate rates of biodegradation in the presence and absence of natural sediment materials. It also may provide limited information on the abiotic degradation rate, and sorption to sediment and vessel walls.

1.3 This test method allows for the development of a first-order rate constant, based on the disappearance of the test compound with time, and a second-order rate constant, normalized for changes in microbial biomass.

1.4 This test method requires a chemical specific analytical method and the concentrations of test substance employed are dependent on the sensitivity of the analytical method.

1.5 This test method is designed to be applicable to compounds that are not inhibitory to bacteria at the concentrations used in the test m......

ASTM E1279-89(2008) Referenced Document

• ASTM D1193 Standard Specification for Reagent Water*， 1999-04-19 Update
• ASTM D4129 Standard Test Method for Total and Organic Carbon in Water by High Temperature Oxidation and by Coulometric Detection*， 1998-04-19 Update
• ASTM E1194 Standard Test Method for Vapor Pressure
• ASTM E1195 Standard Test Method for Determining a Sorption Constant (Koc) for an Organic Chemical in Soil and Sediments
• ASTM E895 Standard Practice for Determination of Hydrolysis Rate Constants of Organic Chemicals in Aqueous Solutions
• ASTM E896 Standard Test Method for Conducting Aqueous Direct Photolysis Tests *， 1992-04-19 Update

ASTM E1279-89(2008) history

• 1989 ASTM E1279-89(2008) Standard Test Method for Biodegradation By a Shake-Flask Die-Away Method
• 1989 ASTM E1279-89(2001) Standard Test Method for Biodegradation By a Shake-Flask Die-Away Method
• 1989 ASTM E1279-89(1995) Standard Test Method for Biodegradation By a Shake-Flask Die-Away Method