ASTM D6499-07
Standard Test Method for The Immunological Measurement of Antigenic Protein in Natural Rubber and its Products

Standard No.

ASTM D6499-07

Release Date

2007

Published By

American Society for Testing and Materials (ASTM)

Status

Be replaced

Replace By

ASTM D6499-12

Latest

ASTM D6499-18(2023)

Scope

1.1 This test method covers an immunological method to determine the amount of antigenic protein in natural rubber and its products using rabbit antisera specific for natural rubber latex (NRL) proteins. This immunoassay procedure quantitatively measures the level of antigenic latex proteins in solution using an inhibition format. The samples may include glove or other rubber product extracts which have been collected in order to measure the latex protein levels. Although this method detects antigenic proteins, it should not be considered as a measure of allergenic proteins. Correlation of protein/antigen levels with the level of allergenic proteins has not been fully established.

1.2 For the purpose of this test method, the range of protein will be measured in terms of microgram to milligram quantities.

1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

ASTM D6499-07 Referenced Document

• ASTM D4483 Standard Practice for Determining Precision for Test Method Standards in the Rubber and Carbon Black Industries
• ASTM D5712 Standard Test Method for Analysis of Aqueous Extractable Protein in Natural Rubber and Its Products Using the Modified Lowry Method
• ASTM E691 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method

ASTM D6499-07 history

• 2023 ASTM D6499-18(2023) Standard Test Method for Immunological Measurement of Antigenic Protein in Hevea Natural Rubber (HNR) and its Products
• 2018 ASTM D6499-18 Standard Test Method for Immunological Measurement of Antigenic Protein in Hevea Natural Rubber (HNR) and its Products
• 2016 ASTM D6499-16 Standard Test Method for The Immunological Measurement of Antigenic Protein in Natural Rubber and its Products
• 2012 ASTM D6499-12 Standard Test Method for The Immunological Measurement of Antigenic Protein in Natural Rubber and its Products
• 2007 ASTM D6499-07 Standard Test Method for The Immunological Measurement of Antigenic Protein in Natural Rubber and its Products
• 2003 ASTM D6499-03 Standard Test Method for the Immunological Measurement of Antigenic Protein in Natural Rubber and its Products
• 2000 ASTM D6499-00 Standard Test Method for the Immunological Measurement of Antigenic Protein in Natural Rubber and its Products