ASTM E2180-07
Standard Test Method for Determining the Activity of Incorporated Antimicrobial Agent(s) In Polymeric or Hydrophobic Materials

Standard No.

ASTM E2180-07

Release Date

2007

Published By

American Society for Testing and Materials (ASTM)

Status

Be replaced

Replace By

ASTM E2180-07(2012)

Latest

ASTM E2180-24

Scope

1.1 This test method is designed to evaluate (quantitatively) the antimicrobial effectiveness of agents incorporated or bound into or onto mainly flat (two dimensional) hydrophobic or polymeric surfaces. The method focuses primarily on assessing antibacterial activity; however, other microorganisms such as yeast and fungal conidia may be tested using this method.

1.2 The vehicle for the inoculum is an agar slurry which reduces the surface tension of the saline inoculum carrier and allows formation of a "pseudo-biofilm," providing more even contact of the inoculum with the test surface.

Note 1

This test method facilitates the testing of hydrophobic surfaces by utilizing cells held in an agar slurry matrix. This test method, as written, is inappropriate to determine efficacy against biofilm cells, which are different both genetically and metabolically than planktonic cells used in this test.

1.3 This method can confirm the presence of antimicrobial activity in plastics or hydrophobic surfaces and allows determination of quantitative differences in antimicrobial activity between untreated plastics or polymers and those with bound or incorporated low water-soluble antimicrobial agents. Comparisons between the numbers of survivors on preservative-treated and control hydrophobic surfaces may also be made.

1.4 The procedure also permits determination of "shelf-life" or long term durability of an antimicrobial treatment which may be achieved through testing both non-washed and washed samples over a time span.

1.5 Knowledge of microbiological techniques is required for these procedures.

This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

ASTM E2180-07 Referenced Document

• ASTM E1054 Standard Practices for Evaluation of Inactivators of Antimicrobial Agents
• ASTM E1428 Standard Test Method for Evaluating the Performance of Antimicrobials in or on Polymeric Solids Against Staining by Streptoverticillium reticulum (A Pink Stain Organism)
• ASTM G21 Standard Practice for Determining Resistance of Synthetic Polymeric Materials to Fungi

ASTM E2180-07 history

• 2024 ASTM E2180-24 Standard Test Method for Determining the Activity of Incorporated Antimicrobial Agent(s) In Polymeric or Hydrophobic Materials
• 2018 ASTM E2180-18 Standard Test Method for Determining the Activity of Incorporated Antimicrobial Agent(s) In Polymeric or Hydrophobic Materials
• 2017 ASTM E2180-07(2017) Standard Test Method for Determining the Activity of Incorporated Antimicrobial Agent(s) In Polymeric or Hydrophobic Materials
• 2007 ASTM E2180-07(2012) Standard Test Method for Determining the Activity of Incorporated Antimicrobial Agent(s) In Polymeric or Hydrophobic Materials
• 2007 ASTM E2180-07 Standard Test Method for Determining the Activity of Incorporated Antimicrobial Agent(s) In Polymeric or Hydrophobic Materials
• 2001 ASTM E2180-01 Standard Test Method for Determining the Activity of Incorporated Antimicrobial Agent(s) In Polymeric or Hydrophobic Materials