ASTM E2250-02
Standard Method for Determination of Endotoxin Concentration in Water Miscible Metal Working Fluids

Standard No.

ASTM E2250-02

Release Date

2002

Published By

American Society for Testing and Materials (ASTM)

Status

Withdraw 2008-07

Latest

ASTM E2250-02

Scope

1.1 This method covers quantitative methods for the sampling and determination of Gram-negative bacterial endotoxin concentrations in water miscible metalworking fluids (MWF).

1.2 Users of this method should be familiar with the handling of MWF.

1.3 This method gives an estimate of the endotoxin concentration of the sampled MWF.

1.3.1 Used on site, this method gives an indication of changes in Gram-negative bacterial contamination in the MWF.

1.3.2 This method does not replace Practice E 2144.

1.4 This method seeks to minimize inter-laboratory variation but does not ensure uniformity of results.

1.5 This method is not intended to relate endotoxin concentration in MWF to health effects of inhaled endotoxin.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

ASTM E2250-02 Referenced Document

• ASTM D1356 Standard Terminology Relating to Sampling and Analysis of Atmospheres*， 2024-04-19 Update
• ASTM D4840 Standard Guide for Sample Chain-of-Custody Procedures
• ASTM E1497 Standard Practice for Selection and Safe Use of Water-Miscible and Straight Oil Metal Removal Fluids
• ASTM E1542 Standard Terminology Relating to Occupational Health and Safety
• ASTM E2144 Standard Practice for Personal Sampling and Analysis of Endotoxin in Metalworking Fluid Aerosols in Workplace Atmospheres

ASTM E2250-02 history

• 2002 ASTM E2250-02 Standard Method for Determination of Endotoxin Concentration in Water Miscible Metal Working Fluids