ASTM D6530-00(2006)
Standard Test Method for Total Active Biomass in Cooling Tower Waters (Kool Kount Assay; KKA)

Standard No.

ASTM D6530-00(2006)

Release Date

2000

Published By

American Society for Testing and Materials (ASTM)

Status

Be replaced

Replace By

ASTM D6530-00(2013)

Latest

ASTM D6530-19

Scope

This test method is useful for rapid determination of viable active biomass concentrations in cooling tower waters. The efficiency of cooling towers is directly affected by the concentration of biomass in the cooling tower waters. As biomass concentrations increase, biofilm formation occurs resulting in a decrease in the efficiency of heat exchange in the tower. Current tests for monitoring the biomass concentration in cooling towers require at least 36 h for growth of the microorganisms on a solid agar surface for counting. Replication of microorganisms over the 36-h period before results are available creates an aqueous environment which is no longer represented by the data generated. Timely test results can assist in minimizing biocide addition to control biomass concentrations. Kool Kount provides data within hours to allow for more precise control of active biomass concentrations in the waters.

1.1 This test method covers the determination of viable active biomass in cooling tower water in the range from 10² to 10⁸ cfu/mL (1). It is a semiquantitative test method.

1.2 This test method was used successfully with reagent water, physiologic saline, and cooling tower waters. It is the user's responsibility to ensure the validity of this test method for waters of untested matrices.

This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific hazard statements, see Section .

ASTM D6530-00(2006) Referenced Document

• ASTM D1129 Standard Terminology Relating to Water
• ASTM D1192 Standard Guide for Equipment for Sampling Water and Steam in Closed Conduits
• ASTM D1193 Standard Specification for Reagent Water
• ASTM D3370 Standard Practices for Sampling Water from Closed Conduits

ASTM D6530-00(2006) history

• 2019 ASTM D6530-19 Standard Test Method for Total Active Biomass in Cooling Tower Waters (Kool Kount Assay; KKA)
• 2000 ASTM D6530-00(2013) Standard Test Method for Total Active Biomass in Cooling Tower Waters lpar;Kool Kount Assay; KKArpar;
• 2000 ASTM D6530-00(2006) Standard Test Method for Total Active Biomass in Cooling Tower Waters (Kool Kount Assay; KKA)
• 2000 ASTM D6530-00 Standard Test Method for Total Active Biomass in Cooling Tower Waters (Kool Kount Assay; KKA)