The standard applies to aqueous solutions of substances of known composition in a standardized medium. The scope of application can be extended to undiluted or slightly diluted waste water if the approximately contents of Na+, K+, Ca2 + , Mg2 + , Cl~, S042~, HC03_ are known and, if necessary, these can be adapted to those of the standardized medium . Note -j- + 2+ 2+ _ 2_ _ The tolerance of Daphnia magna to the combination of Na , K , Ca , Mg , Cl , SO4 and HCO3 is not known. If the levels of these in waste water, etc., are much higher than in the standardized medium, Daphnia magna cannot be used as a test animal.
NEN 6501-1980 history
1980NEN 6501-1980 Water - Determination of acute toxicity with Daphnia magna