ISO 5815-1:2003 Water quality - Determination of biochemical oxygen demand after n days (BODn) - Part 1: Dilution and seeding method with allythiourea addition
This part of ISO 5815 specifies a determination of the biochemical oxygen demand of waters by dilution and seeding with suppression of nitrification.
This part of ISO 5815 is applicable to all waters having biochemical oxygen demands greater than or equal to 3 mg/l of oxygen (the limit of determination) and not exceeding 6 000 mg/l of oxygen. For biochemical oxygen demands greater than 6 000 mg/l of oxygen, the method is still applicable, but the errors caused by the necessary dilutions can influence the analytical quality of the test method and the results are to be interpreted with circumspection.
The results obtained are the product of a combination of biochemical and chemical reactions. They do not have the rigorous and unambiguous character of those resulting from, for example, a single, well-defined, chemical process. Nevertheless, they provide an indication from which the quality of waters can be estimated.
The test can be influenced by the presence of various substances. Those which are toxic to microorganisms, for example bactericides, toxic metals or free chlorine, will inhibit biochemical oxidation. The presence of algae or nitrifying microorganisms can produce artificially high results.
Annex A describes alternative incubation periods.
Annex B describes multitesting, which can be used to obtain enhanced precision or to demonstrate the presence of substances toxic to microorganisms.
Annex C provides precision data.
ISO 5815-1:2003 Referenced Document
ISO 3696:1987 Water for analytical laboratory use; Specification and test methods
ISO 5813:1983 Water quality; Determination of dissolved oxygen; Iodometric method
ISO 5814:1990 Water quality; determination of dissolved oxygen; electrochemical probe method
ISO 6060:1989 Water quality; determination of the chemical oxygen demand
ISO 8245:1999 Water quality - Guidelines for the determination of total organic carbon (TOC) and dissolved organic carbon (DOC)
ISO 8467:1993 Water quality; determination of permanganate index
ISO 5815-1:2003 history
2019ISO 5815-1:2019 Water quality — Determination of biochemical oxygen demand after n days (BODn) — Part 1: Dilution and seeding method with allylthiourea addition
2003ISO 5815-1:2003 Water quality - Determination of biochemical oxygen demand after n days (BOD<(Index)n>) - Part 1: Dilution and seeding method with allythiourea addition