ISO 3594:1976
Milk fat; Detection of vegetable fat by gas-liquid chromatography of sterols (Reference method)
- Standard No.
- ISO 3594:1976
- Release Date
- 1976
- Published By
- International Organization for Standardization (ISO)
- Status
- Latest
- ISO 3594:1976
- Scope
- The method consists in preparing of sterol digitonides and dissolving in a mixture of formamide and dimethylformamide, extraction of the liberated sterols with pentane, separation of the sterols using a suitable gas chromatograph. If, on the chromatogram, a peak with the retention time of beta-sitosterol is obtained, the presence of vegetable fat in the sample is demonstrated. Peaks of other phytosterols may support this conclusion.
ISO 3594:1976 history
- 1976 ISO 3594:1976 Milk fat; Detection of vegetable fat by gas-liquid chromatography of sterols (Reference method)
