DIN EN 13368-2:2007 Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 2: Determination of Fe chelated by o,o-EDDHA and o,o-EDDHMA by ion pair chromatography English version of DIN EN 13368-2:2007-06
This document specifies a method for the chromatographic determination of the iron chelated by each individual ortho(hydroxy)-ortho(hydroxy) isomer of the chelating agents o,o-EDDHA and o,oEDDHMA in fertilizers containing one or both of these substances. The method allows the identification and the determination of the total concentration of water soluble iron chelates of these chelating agents.lt does not determine the free form of the chelating agents.
NOTE 1 The substances EDDHA(ethylene diamine di(hydroxy phenyl) acetic acid)and EDDHMA(ethylene diaminedi(hydroxy methyl phenyl)acetic acid) exist as several different isomeric forms. Positional isomers for the hydroxyl ormethyl groups(in ortho,meta,and para positions)as well as stereo isomers(meso and dl-racemic forms)are known.Bothmeso and dl-racemic forms of the ortho,ortho-EDDHA andortho,ortho-EDDHMA are positional isomers for the hydroxyl groups allowed by the Regulation(EC) No 2003/2003. Since para,method here described the para,meta and ortho methyl positional isomers of the o,o-EDDHMA are considered together.
NOTE 2 At present,analyticallypure standards only exist for ortho,ortho-EDDHA and ortho,ortho-EDDHAM.All other substances being unavailable as a standard,the influence of their eventual presence in the samples(with respect to the sensitivity and the selectivity of this method)has not been studied.
NOTE 3 The meso and the dl-racemic forms of o,o-EDDHA and o,o-EDDHMA can be determined separately by this method.
This procedure concerns EC fertilizers covered by Regulation (EC)No 2003/2003［5］.lt is applicable to a mass fraction of the metal chelated of at least 0,625﹪.
DIN EN 13368-2:2007 history
2018DIN EN 13368-2:2018-03 Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 2: Determination of Fe chelated by [o,o] EDDHA, [o,o] EDDHMA and HBED, or the amount of chelating agents, by ion pair chromatography; German version EN 13368-2:2017
2018DIN EN 13368-2:2018 Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 2: Determination of Fe chelated by [o,o] EDDHA, [o,o] EDDHMA and HBED, or the amount of chelating agents, by ion pair chromatography
1970DIN EN 13368-2 E:2016-07 Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 2: Determination of Fe chelated by [o,o] EDDHA, [o,o] EDDHMA and HBED, or the amount of chelating agents, by ion pair chromatography
2016DIN EN 13368-2 E:2016 Draft Document - Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 2: Determination of Fe chelated by [o,o] EDDHA, [o,o] EDDHMA and HBED, or the amount of chelating agents, by ion pair chromatography; German and En...
2012DIN EN 13368-2:2012 Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 2: Determination of Fe chelated by o,o-EDDHA, o,o-EDDHMA and HBED by ion pair chromatography; German version EN 13368-2:2012
2007DIN EN 13368-2:2007 Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 2: Determination of Fe chelated by o,o-EDDHA and o,o-EDDHMA by ion pair chromatography English version of DIN EN 13368-2:2007-06