ISO 4831:2006 Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of coliforms - Most probable number technique
International Organization for Standardization (ISO)
Latest
ISO 4831:2006
Scope
This International Standard gives general guidelines for the detection and the enumeration of conforms. It is applicable to
— products intended for human consumption and for the feeding of animals, and
— environmental samples in the area of food production and food handling.
Enumeration is carried out by calculation of the most probable number (MPN) after incubation in a liquid medium at 30 °C or 37 °C.
NOTE The temperature is subject to agreement between the parties concerned. In the case of milk and milk products, the temperature of incubation is 30 "C.
This enumeration method is applicable when the number sought is expected to be in the range 1 to 100 per millilitre or per gram of test sample.
A limitation on the applicability of this International Standard is imposed by the susceptibility of the method to a large degree of variability. The information given in Clause 11 provides guidance on the applicability of the method and on the interpretation of the results.
ISO 4831:2006 Referenced Document
ISO 6887 Microbiology - General Guidance for the Preparation of Dilutions for Microbiological Examination First Edition
ISO 7218 Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations AMENDMENT 1*, 2007-08-15 Update
ISO/TS 11133-1 Microbiology of food and animal feeding stuffs - Guidelines on preparation and production of culture media - Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory*, 2009-02-01 Update
ISO/TS 11133-2:2003 Microbiology of food and animal feeding stuffs - Guidelines on preparation and production of culture media - Part 2: Practical guidelines on performance testing of culture media
ISO 4831:2006 history
2006ISO 4831:2006 Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of coliforms - Most probable number technique
1991ISO 4831:1991 Microbiology; general guidance for the enumeration of coliforms; most probable number technique
1978ISO 4831:1978 Microbiology — General guidance for the enumeration of coliforms — Most probable number technique at 30 degrees C