International Organization for Standardization (ISO)
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ISO 4832:2006
Scope
This International Standard gives general guidelines for the enumeration of coliforms. It is applicable to
— products intended for human consumption and for the feeding of animals, and
— environmental samples in the area of food production and food handling,
by means of the technique of counting colonies after incubation on a solid medium at 30℃ or at 37℃.
NOTE The temperature is subject to agreement between the parties concerned. In the case of milk and milk products, the
temperature of incubation is 30℃.
This technique is recommended when the number of colonies sought is expected to be more than 100 per
millilitre or per gram of the test sample.
ISO 4832:2006 Referenced Document
ISO 7218 Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations AMENDMENT 1*, 2007-08-15 Update
ISO/TS 11133-1 Microbiology of food and animal feeding stuffs - Guidelines on preparation and production of culture media - Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory*, 2009-02-01 Update
ISO/TS 11133-2:2003 Microbiology of food and animal feeding stuffs - Guidelines on preparation and production of culture media - Part 2: Practical guidelines on performance testing of culture media
ISO 4832:2006 history
2006ISO 4832:2006 Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of coliforms - Colony-count technique
1991ISO 4832:1991 Microbiology; general guidance for the enumeration of coliforms; colony count technique
1978ISO 4832:1978 Microbiology — General guidance for enumeration of coliforms — Colony count technique at 30 degrees C