ANSI/ASTM D5296:1997 Molecular Weight Averages and Molecular Weight Distribution of Polystyrene by High Performance Size-Exclusion Chromatography, Test Method of (08.03)
This test method covers the determination of molecular weight (MW) averages and the distribution of molecular weights for linear, soluble polystyrene by liquid high-performance size-exclusion chromatography (HPSEC). This test method is not absolute and requires the use of commercially available narrow molecular weight distribution (MWD) polystyrene standards for calibration. This test method is applicable for samples containing molecular weight components that have elution volumes falling within the elution volume range defined by polystyrene standards (that is, molecular weights generally from 2000 to 2 000 000 gmol<(hoch)1>). The HPSEC is differentiated from traditional size-exclusion chromatography SEC (also referred to as gel permeation chromatography (GPC)) in that the number of theoretical plates per metre with an HPSEC system is about ten times greater than that for traditional SEC (see Terminology D 883 and Practice D 3016). The HPSEC systems employ low-volume liquid chromatography components and columns packed with relatively small (generally 3 to 20 m) microporous particles. High-performance liquid chromatography instrumentation and automated data handling systems for data acquisition and processing are required. The values stated in SI units are to be regarded as the standard. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Section .Note 1There is no similar or equivalent ISO standard.
ANSI/ASTM D5296:1997 history
1997ANSI/ASTM D5296:1997 Molecular Weight Averages and Molecular Weight Distribution of Polystyrene by High Performance Size-Exclusion Chromatography, Test Method of (08.03)