BS EN ISO 5509:2001
Animal and vegetable fats and oils - Preparation of methyl esters of fatty acids

Standard No.

BS EN ISO 5509:2001

Release Date

2001

Published By

British Standards Institution (BSI)

Status

Be replaced 2011-03

Replace By

BS EN ISO 12966-2:2011

Latest

BS EN ISO 12966-2:2017

Scope

This International Standard specifies methods of preparing the methyl esters of fatty acids. It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements, three methylation methods are specified, as follows: a) boron trifluoride (BF3) method (see clause 3); b) trimethylsulfonium hydroxide (TMSH) method (see clause 4); c) trans-esterification method (see clause 5). Methyl esters so produced are used in various analytical procedures requiring such derivatives, for example gas-liquid chromatography (GLC), thin-layer chromatography (TLC) and infrared spectrometry (IR).

BS EN ISO 5509:2001 history

• 2017 BS EN ISO 12966-2:2017 Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters. Preparation of methyl esters of fatty acids
• 2011 BS EN ISO 12966-2:2011 Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters. Preparation of methyl esters of fatty acids
• 2001 BS EN ISO 5509:2001 Animal and vegetable fats and oils - Preparation of methyl esters of fatty acids

BS EN ISO 5509:2001 Animal and vegetable fats and oils - Preparation of methyl esters of fatty acids was changed to BS EN ISO 12966-2:2011 Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters. Preparation of methyl esters of fatty acids.